山东农业科学2016，Vol.48Issue(6)：7-10,4.DOI:10.14083/j.issn.1001-4942.2016.06.002
大白菜抗根肿病基因 CRb 的分子标记验证与种质资源筛选
Identification of Molecular Markers for Clubroot Disease Resistant Gene CRb and Screening of Germplasm Resources of Chinese Cabbage
摘要
Abstract
Clubroot disease,caused by Plasmodiophora brassicae,is one of soil -borne disease seriously affecting the growth and development of Chinese cabbage (Brassica pekinensis L.)and has become one of the most serious diseases.Gene CRb is one of the most important gene against to clubroot disease in Chinese cab-bage.In this research,TCR05,an effective codominant marker closely linked to CRb,was identified from 6 published molecular markers.The marker owned a 279 -bp PCR amplification fragment in the resistant lines, a 250 -bp fragment in the susceptible lines and both 279 -bp and 250 -bp fragments in the heterozygous combinations.TCR05 was used to screen 24 germplasms.It was proved that they all contained gene CRb,8 were homozygous and the others were heterozygous.The results were in accordance with the field test results.关键词
大白菜/根肿病/CRb/分子标记/种质资源Key words
Chinese cabbage/Clubroot disease/CRb/Molecular marker/Germplasm resource分类
农业科技引用本文复制引用
任平平,马安峰,程斐,孙朝辉,高建伟..大白菜抗根肿病基因 CRb 的分子标记验证与种质资源筛选[J].山东农业科学,2016,48(6):7-10,4.基金项目
山东省现代农业产业技术体系项目（SDAIT －02－022－04）；山东省农业良种工程项目“十字花科名产蔬菜新品种选育”［鲁科字（2014）96号］ （）
