山东农业科学2016，Vol.48Issue(6)：105-108,4.DOI:10.14083/j.issn.1001-4942.2016.06.026
T 细胞表位多肽疫苗对禽流感病毒的免疫保护评价
Immune Protection Evaluation of T Cell Epitope Peptide Vaccine for Avian Influenza Virus
摘要
Abstract
To explore the effect of T cell epitope peptide NP67 -74 -KLH of NP protein on immune effect of 4M2e - MAP,the BALB /c mice were immunized by NP67 -74 -KLH and 4M2e - MAP after adding the complete freund’s adjuvant and incomplete freund’s adjuvant to evaluate the immune effect.The weight change curve of mice after challenge showed that the weight change trend of mice immunized combinedly by NP67 -74 -KLH and 4M2e -MAP was more smooth than that immunized by 4M2e -MAP,and the weight of mice in combined immunization group regained quickly.The results of fluorescent quantitation and lung tissue pathological slices showed that the compound polypeptide of NP67 -74 -KLH with 4M2e -MAP could inhibit the virus replication in the lungs,reduce the pathological damage to lungs and get the effective protection dur-ing the course of resisting virus.This provided a good reference for the combined use of peptide vaccine a-gainst avian influenza research.关键词
禽流感/多肽疫苗/免疫效果Key words
Avian influenza/Polypeptide vaccine/Immune effect分类
农业科技引用本文复制引用
朱凤珠,许传田,鲁梅,谭刘刚,孔正杰,黄庆华,黄艳艳,杨少华,张秀美,崔言顺..T 细胞表位多肽疫苗对禽流感病毒的免疫保护评价[J].山东农业科学,2016,48(6):105-108,4.基金项目
国家公益性行业（农业）科研专项（201303033）；现代农业产业技术体系 （）
