沈阳医学院学报2016，Vol.18Issue(3)：149-150,153,3.DOI:10.16753/j.cnki.1008-2344.2016.03.007
类透明质酸壳聚糖微乳和碳铂联合给药对大鼠胶质瘤的治疗效果评价
Therapeutic Effect of Hyaluronic Acid Chitosan-based Microemulsion and Carboplatin on Glioma in Rats
摘要
Abstract
Objective: To study the therapeutic effect of hyaluronic acid chitosan⁃based microemulsion ( HAC⁃ME) and carboplatin in a Wistar rat model bearing cerebral C6 glioma xenografts. Methods:C6 rat glioma cells were cultured normally. A total of 30 Wistar rats bearing orthotopic C6 glioma xenografts were randomly divided into 3 groups, and administrated with physiological saline, carbopl⁃atin or HAC⁃ME and carboplatin in each group. There are 10 rats in each group. The rat apoptosis changes and survival time were ob⁃served after treated with physiological saline, carboplatin or HAC⁃ME and carboplatin in each group. Results:Glioma cells were nega⁃tive in saline group with TUNEL staining, the nuclei of individual glioma cells in glioma tissue were stained with brown, indicating ap⁃optosis occur in carboplatin group, the apoptosis of glioma cells in glioma tissue significantly increased in HAC⁃ME and carboplatin group with TUNEL staining. The survival time in HAC⁃ME and carboplatin group was longer than that in saline group and carboplatin group ( P<0�05) . Conclusion:Administration of HAC⁃ME and carboplatin can suppress the growth of C6 glioma in rats and may pro⁃vide experimental basis for clinical treatment of glioma.关键词
类透明质酸壳聚糖微乳/胶质瘤/碳铂/生存期Key words
hyaluronic acid chitosan-based microemulsion/glioma/carboplatin/survival time分类
医药卫生引用本文复制引用
陆威成,解辉,丁晓慧,祁荣,邢海会,吴昊澎..类透明质酸壳聚糖微乳和碳铂联合给药对大鼠胶质瘤的治疗效果评价[J].沈阳医学院学报,2016,18(3):149-150,153,3.基金项目
辽宁省科技厅博士启动基金项目（ No?20131114）；辽宁省教育厅科学技术研究一般项目（ No?L2015535）；沈阳医学院科技基金项目 （）
