特产研究2016，Vol.38Issue(2)：6-11,6.DOI:10.16720/j.cnki.tcyj.2016.02.002
鉴别PRRSV野毒感染与弱毒活疫苗TJM－F92免疫d120－ELISA方法的应用
Application of d120-ELISA for Discrimination of Animals Infected with Wide -Type PRRSV from Those Vaccinated
王西西 1王凤雪 1李真光 1温永俊 1王鑫 2师新川 2宋妮 2武华1
作者信息
- 1. 中国农业科学院特产研究所特种经济动物分子生物学国家重点实验室，长春 130112
- 2. 华威特北京生物科技有限公司，北京 100085
- 折叠
摘要
Abstract
This study was designed to describe the characters of antibody kinetics of N and GST -d120 proteins and distinguish pigs infection with wild type virus from those vaccinated with modified live vaccine TJM -F92 or other inactivated vaccines on different immune states based on the clinical sera S/P values tested by both the d120-ELISA and IDEXX .Serum samples were collected from experimental animals and clin-ical animals .Results of kinetics of the antibody responses against N and GST -d120aa proteins indicate that the d120-ELISA can distinguish animals infected with wild-type PRRSV TJ infection from vaccinated ones .The d120-ELISA can detect JXA1-R maternal antibodies from those vaccinated with TJM -F92 ,and it cannot detect antibodies against modified live vaccine JXA 1-R at 14 days but at 28 days the positive rate of it is 13/15 (86.7% ) with high sera S/P values .Two farms where pigs were vaccinated with inactivated vaccine ,the positive rates of sera from the farm infected with PRRSV by d120-ELISA and IDEXX were 9/20(45.0% ) and 20/20(100% ) ,while the other's were 0/30(0% ) and 12/30 (40% ) .In conclude ,the d120 ELISA can differentiate pigs infected with wild -type PRRSV or vaccinated with JXA1-R from those vaccinated with TJM -F92 or inactivated vaccines .关键词
猪繁殖与呼吸综合征/非结构蛋白2/d120-ELISA/血清动力学/应用Key words
PRRSV/non-structural protein 2/d120-ELISA antibody kinetics experiment/Application分类
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王西西,王凤雪,李真光,温永俊,王鑫,师新川,宋妮,武华..鉴别PRRSV野毒感染与弱毒活疫苗TJM－F92免疫d120－ELISA方法的应用[J].特产研究,2016,38(2):6-11,6.
