西安交通大学学报（医学版）2016，Vol.37Issue(4)：513-517,524,6.DOI:10.7652/jdyxb201604009
低氧诱导因子在肝肺综合征大鼠肺组织中的表达及其与糖调节蛋白78的关系
Pulmonary expression of HIF-1α and its relationship with GRP78 in the pathogenesis of hepatopulmonary syndrome in rats
摘要
Abstract
ABSTRACT:Objective To explore the role of HIF‐1αin the pathogenesis of hepatopulmonary syndrome (HPS) and its relationship with GRP78 .Methods The HPS model in rats was induced by multiple pathogenic factor .The samples were assessed by using Western blotting analysis for HIF‐lα, GRP78 and VEGF164 . The expressions of VEGFR‐2 and CD105 were observed by using immunohistochemical staining .Results The protein level of HIF‐1αwas significantly increased in HPS group at week 8 compared with that at week 4 and 6 groups and corresponding normal control groups .With the development of HPS ,protein level of GRP78 was gradually increased at each time point significantly and reached the highest level at week 8 ;protein level of VEGF164 showed a similar change with GRP78 ,but the peak was at week 6 .Immunohistochemical results showed that the protein expressions of VEGFR‐2 and CD105 were gradually increased in lung tissue as HPS progressed .The protein level of GRP78 was positively correlated with HIF‐1α,VEGF164 ,VEGFR‐2 and CD105 ,respectively (P<0 .05) .Conclusion HIF‐1αis most likely together with GRP78 to play a critical role in promoting pulmonary microvascular remodeling in the pathogenesis of HPS in rats .关键词
肝肺综合征/低氧诱导因子-1α/糖调节蛋白78/肝硬化/血管内皮生长因子Key words
hepatopulmonary syndrome/hypoxia-inducible factor-1α/78kD glucose-regulated protein/liver cirrhosis/vascular endothelial growth factor分类
医药卫生引用本文复制引用
李旭炯,张慧英,田小霞,陈云霞,孟莉,来丽娜,赵中夫,韩德五,Cheng Ji..低氧诱导因子在肝肺综合征大鼠肺组织中的表达及其与糖调节蛋白78的关系[J].西安交通大学学报（医学版）,2016,37(4):513-517,524,6.基金项目
国家自然科学基金资助项目（No ．81070339）；山西省国际科技合作计划项目（No ．2010081068）；山西省回国留学人员科研资助项目（No ．211-091）；山西医科大学细胞生理学省部共建教育部重点实验室主任基金资助项目（No ．2010-09） Supported by the National Natural Science Foundation of China （No ．81070339）， the International Science and Technology Cooperation Project of Shanxi Province （No ．2010081068），Research Project Supported by Shanxi Scholarship Council of China （No ．211-091），the fund from Key Laboratory of Cellular Physiology co-established by Shanxi Province and Ministry of Education in Shanxi Medical University（No ．2010-09）． （）
