烟草科技2016，Vol.49Issue(6)：8-14,7.DOI:10.16135/j.issn1002-0861.20160602
烤烟不同黄瓜花叶病毒病（CMV）抗源的抗性遗传分析
Genetic analysis of CMV resistance in different flue-cured tobacco germplasms
摘要
Abstract
For further understanding the genetic mechanism of cucumber mosaic virus (CMV) resistance in different elite flue-cured tobacco accessions, three inbred lines (Kang88, T.T.8, and FC8) with high resistance to CMV were separately used as paternal parent to construct three F2 populations, while a susceptible inbred line (C151) as the common maternal parent. The disease resistance level was evaluated by the method of mechanical inoculation at seedling stage in greenhouse, and the inheritance was studied with major gene plus polygene mixed genetic model in multiple generations (P1, P2, F1, and F2) from three crosses between the three resistant lines and a susceptible line. The results showed that the resistance in both Kang88 and T.T.8 were controlled by two additive-dominance-epistatic major genes and plus-dominant multiple genes (E1 model), and the heritabilities of major genes were 91.91% and 68.50%, respectively. The inheritance of FC8 was fitted by the two additive-dominance-epistatic major genes mixed model (B1 model), and the heritability of major genes was 76.43%. Because of the high heritability of the major genes in all three resistant lines mentioned above, the selection of resistant plant could be carried out at early stage. The epistatic effects of major genes, as well asthe additive and dominance effects of polygene in both Kang88 and T.T.8, played a positive role in enhancing resistance level, which could be utilized in breeding. The two major genes in FC8 showed significant dominant effects, and were suitable for heterosis utilization.关键词
烤烟/CMV抗性/主基因+多基因/遗传分析Key words
Flue-cured tobacco/Resistance to CMV/Major gene plus polygene/Genetic analysis分类
农业科技引用本文复制引用
程亚增,马冰,蒋彩虹,张兴伟,任民,程立锐,杨爱国,王元英..烤烟不同黄瓜花叶病毒病（CMV）抗源的抗性遗传分析[J].烟草科技,2016,49(6):8-14,7.基金项目
国家烟草专卖局烟草基因组计划重大专项“烟草抗CMV和赤星病连锁分子标记开发及NC82、K326品种抗性改良（110201301009）”；“烟草重要性状基因发掘及功能标记开发（110201301008）”；中国烟草总公司重点科技项目“烟草抗主要病毒病（TMV、CMV、PVY）基础材料创制及育种利用（110201402006）”。 （）
