烟草科技2016，Vol.49Issue(6)：15-21,7.DOI:10.16135/j.issn1002-0861.20160603
河南植烟区烟草黑胫病菌生理小种的鉴定及遗传多样性分析
Genetic diversity analysis and physiological race identification of Phytophthora parasitica var. nicotinanae in Henan tobacco planting areas
摘要
Abstract
To identify the physiological races and genetic diversity of Phytophthora parasitica var. nicotinanae in major tobacco planting areas of Henan Province, twenty-eight physiological races were identified by a laboratory tissue culture method and the genetic diversity analysis of twenty-eight pathogens was conducted by melting curve of RAPD (McRAPD). The results showed that: 1) Twenty-five P. parasitica var. nicotinanae isolates were accurately identified as the 1st physiological race, accounting for 89.2% of tested strains, and the other three strains could not be determined at the present. 2) Tested strains were clustered into six groups according to unweighted pair group method with arithmetic average (UPGMA) and the genetic similarity coefficient of 0.79. Except that several strains clustered into the same group were from the same area or close areas, the genetic variation of most strains did not directly correlated to geographical origins and physiological race types.关键词
河南植烟区/烟草黑胫病菌/生理小种/遗传多样性/RAPD熔解曲线分析法(McRAPD)Key words
Henan tobacco planting area/Phytophthora parasitica var. nicotinanae/Physiological race/Genetic diversity analysis/Melting curve of RAPD(McRAPD)分类
农业科技引用本文复制引用
刘芳,奚家勤,董洪旭,牟文君,马聪,徐敏,宋纪真,胡利伟..河南植烟区烟草黑胫病菌生理小种的鉴定及遗传多样性分析[J].烟草科技,2016,49(6):15-21,7.基金项目
中国烟草总公司河南省公司科技项目“烟草黑胫病防控关键技术研发与应用”（122013DS0320）。 （）
