烟草科技2016，Vol.49Issue(6)：45-49,5.DOI:10.16135/j.issn1002-0861.20160607
卷烟烟气暴露下A549和BEAS-2B细胞促炎性因子的释放变化
Release of proinflammatory cytokines from A549 cells and BEAS-2B cells exposed to mainstream cigarette smoke
摘要
Abstract
In order to compare the differences of response to proinflammatory reaction induced by mainstream cigarette smoke among cells of different types, neutral red uptake (NRU) assay was employed to assess the cytotoxicity of cigarette smoke total particulate matter (TPM) or whole smoke (WS) to A549 cells (human adenocarcinoma cells) and BEAS-2B cells (human bronchial epithelial cells). The levels of proinflammatory cytokines interleukin-6 and interleukin-8 (IL-6 and IL-8) released from A549 and BEAS-2B cells exposed to TPM and WS were analyzed by ELISA. The results showed that BEAS-2B cells were more sensitive to smoke-induced cytotoxicity than A549 cells. The induction of IL-6 and IL-8 released from A549 cells was not impacted by cigarette smoke exposure, while TPM and WS all caused dose-dependently increase of IL-6 and IL-8 released from BEAS-2B cells. The proinflammatory response to cigarette smoke thus might be different depending on cell types.关键词
卷烟烟气/全烟气暴露/烟气总粒相物/促炎性细胞因子/细胞毒性Key words
Cigarette smoke/Whole smoke exposure/Total particulate matter/Proinflammatory cytokine/Cytotoxicity分类
轻工纺织引用本文复制引用
李翔,张世敏,赵俊伟,尚平平,彭斌,谢复炜,刘惠民,谢剑平..卷烟烟气暴露下A549和BEAS-2B细胞促炎性因子的释放变化[J].烟草科技,2016,49(6):45-49,5.基金项目
中国烟草总公司郑州烟草研究院科技项目“基于MRTP健康风险评估框架的体外毒理学测试方法研究”（322013CZ0600）。 （）
