医药导报2016，Vol.35Issue(7)：705-709,5.DOI:10.3870/j.issn.1004-0781.2016.07.006
赖氨甘草次酸增溶及对结肠癌HCT-8细胞增殖抑制作用∗
Solubilization and Inhibitory Action of Glycyrrhetinic Acid Lysinate on Cell Proliferation of Coloretal Cancer Cell Line HCT-8
摘要
Abstract
Objective To prepare,characterize glycyrrhetinic acid lysinate,and study the solubilization and inhibitory action antitumor activity of glycyrrhetinic acid lysinate on cell proliferation of colorectal cancer cell line HCT-8. Methods Glycyrrhetinic acid lysinate was prepared by co-grinding glycyrrhetinic acid with lysine in 1∶1 molar mixture for 10 hours. Characterization of glycyrrhetinic acid lysinate was achieved by X-ray powder diffraction, infrared spectroscopy, and ultraviolet spectrum techniques.HPLC method was used to study the solubilization of glycyrrhetinic acid lysinate.The MTT method was used to assay the inhibitory action of glycyrrhetinic acid lysinate on cell proliferation. Results The solubility of glycyrrhetinic acid lysinate was enhanced 260 folds,compared with glycyrrhetinicacid in water. The inhibitory cell proliferation action on HCT-8 of glycyrrhetinic acid lysinate was 7 times higher than that of glycyrrhetinic acid. Conclusion The satisfactory water solubility and antitumor activity of glycyrrhetinic acid lysinate will be potentially useful for its application as a new pharmaceutical formulation in cancer treatment in the future.关键词
赖氨甘草次酸/增溶/色谱法,高效液相/抗肿瘤活性Key words
Glycyrrhetinic acid lysinate/Solubilization/Chromatography, high performance liquid/Antitumor activity分类
医药卫生引用本文复制引用
张丽娟,钟家亮,古同男,魏秋芬..赖氨甘草次酸增溶及对结肠癌HCT-8细胞增殖抑制作用∗[J].医药导报,2016,35(7):705-709,5.基金项目
北京市教育委员会科技发展计划面上项目( KM200910025014) （ KM200910025014）
