中国比较医学杂志2016，Vol.26Issue(6)：1-5,10,6.DOI:10.3969/j.issn.1671-7856.2016.06.001
游离SIVmac239病毒与T细胞介导两种方式感染内皮细胞的效果比较
Comparison of the SIV infection in endothelial cells by SIVmac239 directly or mediated by T cells
摘要
Abstract
Objective To compare the effects of two approaches of SIVmac239 infection in endothelial cells, directly by cell⁃free virus or by T cell⁃mediated infection, to explore the predominant approach of SIVmac239 infection in endothelial cells, and finally lay a theoretical foundation of the molecular mechanism of SIV viral disruption of the blood⁃brain barrier. Methods We adopted two distinct ways to infect endothelial cells. One was to directly infect the endothelial cells by cell⁃free virus, another was by co⁃culture of infected CEMx174 cells with the endothelial cells. The degree of infection in the endothelial cells was evaluated by nested⁃PCR, indirect immunofluorescence assay, Western blotting and ELISA assays. Results The provirus DNA was found in the endothelial cells infected in either way. However, the endothelial cells infected by co⁃culture with CEMx174 cells had a significantly higher amount of provirus DNA and higher expression of SIV P27 in the cells and in the supernatant than those in the ECs directly infected by cell⁃free virus. Conclusions The way of T cell⁃mediated infection substantially enhances the capacity of SIV infection of endothelial cells than the direct infection by cell⁃free virus. It indicates that the T cell⁃mediated infection may serve as the principal route of SIV disruption of the blood⁃brain barrier.关键词
SIV/艾滋病脑病/内皮细胞/P27Key words
Simian immunodeficiency virus/AIDS encephalopathy/Endothelial cells/P27分类
医药卫生引用本文复制引用
黄丹,丛喆,徐珮,薛婧,魏强..游离SIVmac239病毒与T细胞介导两种方式感染内皮细胞的效果比较[J].中国比较医学杂志,2016,26(6):1-5,10,6.基金项目
国家十二五科技重大专项课题（2012ZX10004-501-001和2013ZX10004-608-003）。 （）
