中国比较医学杂志2016，Vol.26Issue(6)：37-41,47,6.DOI:10.3969/j.issn.1671-7856.2016.06.008
TALEN构建Fndc5基因敲除小鼠及初步分析
Constructtion of a Fndc5 knockout mouse model by TALEN-mediated DNA targeting
摘要
Abstract
Objective To construct Fndc5 knockout mouse models and provide animal models for related studies in the future. Methods Indels were introduced into FNIII domain of Fndc5 gene by TALEN technology in mice, and genotypes were identified by sequencing. To set stable genetic system by pairing. Then at mRNA and DNA levels identified the genetype of born mice. At the same time the body weight and blood glucose of the mice at different ages were analyzed. Finally the Fndc5 expression in the kidney, liver, brain, muscles, heart and other organs was determined by qPCR. Results Four different Fndc5⁃KO lines were generated. The body weight and blood glucose of the mice at different ages showed no significant differences. Finally high Fndc5 expressions in the muscles, heart and other organs were determined. Conclusions We Have for the first time successfully generated Fndc5 knockout ( KO ) mouse model using TALEN mediated DNA targeting technique, and performed preliminary analysis. This Fndc5 knockout ( KO) mouse model provides a novel tool for further studies on the in vivo function of FNDC5.关键词
骨骼肌/Fndc5基因/纤维连接蛋白/TALENKey words
Skeletal muscle/Fndc5/Fibronectin/TALEN/Knockout mouse model分类
医药卫生引用本文复制引用
吴子环,匡世焕,金文,胡雄兵,毛凤仪,王超,张彬,庄峰锋,胡克平,孙晓波,刘晓..TALEN构建Fndc5基因敲除小鼠及初步分析[J].中国比较医学杂志,2016,26(6):37-41,47,6.基金项目
国家自然科学基金面上项目（81471070）；“重大新药创制”科技重大专项（2012ZX09101、2012ZX09301002-001）；诺华诺德－协和英才基金（肌信息素抵抗肥胖作用机理的研究）；药植所创新团队发展计划资助。 （）
