中国比较医学杂志2016，Vol.26Issue(6)：61-66,74,7.DOI:10.3969/j.issn.1671-7856.2016.06.012
新生儿缺氧缺血脑损伤大鼠模型的制备
Preparation of a rat model of neonatal hypoxic-ischemic encephalopathy
摘要
Abstract
Objective To simulate the process of hypoxic⁃ischemic brain injury at high altitude in a simulated cabin with plateau low pressure environment, and to prepare a rat model of cerebral injuries at different high altitudes. Method Thirty⁃two 0⁃day⁃old neonatal SD rats were divided into four groups, namely group A ( control group) and three test groups:group B (2000 m group), group C (4000 m group), and group D (6000 m group). The rats of control group were reared in a barrier environment. The rats of test groups were placed in a simulated cabin with plateau low pressure environment, and to prepare neonatal cerebral ischemia⁃hypoxia model by sport activities. The sport movements were carried out in the cabin in a swimming groove 60 min/d, and not less than 20 hours a day at high altitude low pressure environment. Zea Longa 5 point scale standard was used to determine the behavioral scores at the 3 th 7 th 11 th 15 th days, and samples were collected on the 15th day to observe red blood cell morphology using HE and 2, 3, 5⁃triphenyltetrazolium chloride ( TTC ) staining and ultrastructure by scanning electron microscopy. Result ( 1 ) The neurological scores of the test groups A, B, C were significantly different from that of the control group (P<0�05), and the scores of test group D and control group were very significantly different ( P <0�01 ) . ( 2 ) The histopathological examination using HE staining showed inflammatory cell infiltration in all rats of the test groups, and the extent of inflammatory cell infiltration was positively correlated with the increase of altitude. ( 3 ) The histopathology with TTC staining revealed prominent ischemia in the cerebral cortex of rats in the plateau hypoxic environment. ( 4 ) Scanning electron microscopy showed that the rat erythrocytes were cap⁃like in the group B, irregular in the group C, and zigzag shape in the group D. Conclusions In this study, a rat model of neonatal hypoxic⁃ischemic encephalopathy ( HIE) is successfully established by hypoxic cabin combined with sport activity. This model is stable, reliable, more closely mimicking the pathogenesis and clinical manifestation of neonatal HIE than models prepared with other methods, therefore, may be used in related research.关键词
新生儿脑缺氧缺血性脑病/TTC染色/扫描电镜/大鼠Key words
Neonatal hypoxic-ischemic encephalopathy, Hypoxic-ischemic encephalophathy, HIE/2, 3, 5-triphenyltetrazolium chloride, TTC/Histopathology/Scanning electron microscopy/Rats分类
医药卫生引用本文复制引用
庞炜,曹帅帅,李树祎,田雨光,顾为望..新生儿缺氧缺血脑损伤大鼠模型的制备[J].中国比较医学杂志,2016,26(6):61-66,74,7.基金项目
高新区发展引导专项（2012B011000004）；广东省科技基础条件建设项目（2012B060300003）；高新区发展引导专项（2010A011200003）；广东省科技基础条件建设项目（2013B030300040）；广东省科技基础条件建设项目（2013B060300001）；广东省科技基础条件建设项目（2010B060500001）；国家国际科技合作专项项目（2011DFA33290）。 （）
