中国医学创新2016，Vol.13Issue(17)：8-10,11,4.DOI:10.3969/j.issn.1674-4985.2016.17.002
IFNα-2b对白血病K562细胞增殖及FAK mRNA表达的影响
Effects of Interferon α-2b on Cell Multiplication and FAK mRNA Expression in Leukemia K562 Cells
摘要
Abstract
Objective:To discuss recombinant human interferon (IFNα-2b) on human chronic myelogenous leukemia cell acute transformation phase cell line K562 cell proliferation and focal adhesion kinase (FAK) mRNA expression.Method:The inhibitory rate of IFNα-2b on K562 cells was detected by MTT method and the effect ofα-2b on the expression level of mRNA FAK in K562 cells was detected by RT-PCR technique. Result:In addition to 48 h and 72 h,the ratio of inhibiting K562 cell proliferation increased gradually by increasing IFNα-2b concentration and prolonging reaction time.IFNα-2b in 48 h and the concentration 10 thousands U/mL was the highest to inhibit cell growth.With the increase of the IFNα-2b concentration,FAK mRNA expression was increased by K562 cell,the highest expression level was experimental FAK mRNA of 10 thousands U/mL IFNα-2b,the differences were statistically significant of each groups(P<0.05). Conclusion:The abnormal expression of FAK mRNA in leukemia K562 cells,IFNα-2b may inhibit the proliferation of K562 cells by up regulating the expression level of normal mRNA FAK.关键词
IFNα-2b/K562细胞/FAKKey words
Interferonα-2b/K562 cells/Focal adhesion kinase引用本文复制引用
刘小利,陈日玲..IFNα-2b对白血病K562细胞增殖及FAK mRNA表达的影响[J].中国医学创新,2016,13(17):8-10,11,4.基金项目
广东省社会发展领域科技计划项目 （）
