中国医学创新2016，Vol.13Issue(17)：11-14,4.DOI:10.3969/j.issn.1674-4985.2016.17.003
SIRT1选择性剪接变异体SIRT1-ΔExon8 mRNA在幼龄和老龄小鼠海马组织的表达改变及意义
Expression Changes and Significance of SIRT1 Alternative Splicing Variants of SIRT1- Exon8 mRNA in the Hippocampus of Young and Aged Mice
摘要
Abstract
Objective:To detect whether existence of SIRT1 alternatively spliced variants SIRT1-ΔExon8 and its mRNA expression level in young and aged mice hippocampus.Method:A total of 10 cases of 50 days (young)and 10 cases of 12 age (aging)Kunming (KM) male mice were selected,reverse transcription and PCR were carried by extracting RNA from the hippocampus tissue,SIRT1-ΔExon8 mRNA in hippocampus tissue were detected by agarose gel electrophoresis and SIRT1-ΔExon8 mRNA expression were detected by Real-time PCR (RT-PCR) technology.Result:Young and aging mice hippocampus tissue all detected SIRT1-ΔExon8 mRNA. Compared with the young group,SIRT1-ΔExon8 increased expression of mRNA in aging mice. Conclusion:SIRT1 alternative splicing variant of SIRT1-ΔExon8 mRNA expression in aged mice hippocampus tissue is increased than young mice,thus SIRT1-ΔExon8 may be involved in the occurrence of aging,point out regulating the expression of SIRT1-ΔExon8 may delay aging.关键词
SIRT1-ΔExon8/选择性剪接/海马/衰老Key words
SIRT1-ΔExon8/Alternative splicing/Hippocampal/Aging引用本文复制引用
高瑞君,李艳丽,王锐,高杨,何泽平,杨小荣..SIRT1选择性剪接变异体SIRT1-ΔExon8 mRNA在幼龄和老龄小鼠海马组织的表达改变及意义[J].中国医学创新,2016,13(17):11-14,4.基金项目
国家青年科学基金资助项目（31000481）；山西省高等学校优秀青年学术带头人支持计划资助项目 （）
