中国医学创新2016，Vol.13Issue(17)：19-22,4.DOI:10.3969/j.issn.1674-4985.2016.17.005
大鼠前扣带回皮层吻侧部精确取样的方法
The Method of Taking the Rostral Anterior Cingulate Cortex Tissue Precisely from Rat Brain
摘要
Abstract
Objective:To take the rostral anterior cingulate cortex (rACC) tissue precisely from rat brain with home-made rat brain cutting mold.Method:(1)The cutting mold was made by ourselves.(2)The rat’s brain was cut into coronary sections at low temperature.(3)After that,the tissue section was put on the home-made cutting mold according to the marked line,it might be cut step by step. We finally got accurate sample size at exact location from rACC brain area in rat.After the above,the following studies on the detection of protein or nucleic acid may proceed.Result:In this way,we got accurate rACC brain area sample tissues which were in the same size and location in rat, regardless of different individual experimental animals we used or each new experiment operator.Conclusion:Home-made cutting mold is easy to make and use, and it can be well applied to other brain area in rat or other small animals.关键词
前扣带回皮层吻侧部/脑组织/自制取材模具/精确取样/大鼠Key words
Rostral anterior cingulate cortex/Brain tissue/Home-made cutting mold/Taking accurate sample size at exact location/Rat引用本文复制引用
王媛,李建国,张策,张宇,秦霞,陈建鸣,王锐,赵欣,彭源舒,马洋,秦国华,陈晋源..大鼠前扣带回皮层吻侧部精确取样的方法[J].中国医学创新,2016,13(17):19-22,4.基金项目
国家自然科学基金资助项目 （）
