中国医学创新2016，Vol.13Issue(17)：23-25,3.DOI:10.3969/j.issn.1674-4985.2016.17.006
精液液化相关基因单核苷酸多态性研究
Semen Liquefaction Related Gene Single Nucleotide Polymorphisms Research
摘要
Abstract
Objective:To found that 2-3 PSA gene SNP markers associated with abnormal semen liquefaction,for the future clinical early diagnosis of congenital abnormal semen liquefaction provides a new diagnostic method,in order to further study the molecular mechanism of semen liquefaction which provides the theoretical foundation and treatment of abnormal semen liquefaction.Method:From January 1, 2015 to May 30, 100 male patients in our reproductive center were selected,according to the semen liquefaction time were divided into control group and study group,each group had 50 cases,the peripheral blood DNA of the two groups as a template,PCR amplification of PSA gene sequencing,after five exons by sequencing software analysis results, looking for the control group and the team PSA gene single nucleotide polymorphism loci(SNP).Result:Five PSA gene exon sino-foreign show son 2 mutations and mutation of exon 3,other exons without the mutation,the control group and the team exon 2 and 3 detection comparative differences had no statistical significance(P>0.05). The control group compared with the study group alleles and frequency difference had no statistical significance (P>0.05).Exon 2 genotype of the control group had CC, CT, TT, genotype frequency were 0,62.0%,36.0%, respectively,exon 2 genotype of the study group had CC,CT,TT,genotype frequency were 0,72.0%,28.0%, respectively,comparing differences between two groups had no statistical significance(P>0.05).Conclusion:In the control group and research group of semen liquefaction and PSA genes there is no correlation, these mutations may be PSA genes of these mutations have not been able to cause the change of the PSA protein expression,the causes of abnormal semen liquefied remains to be further research.关键词
前列腺特异性抗原基因/单核苷酸多态性/精液液化Key words
The prostate specific antigen gene/Single nucleotide polymorphisms/Semen liquefaction引用本文复制引用
肖素梅,甘建玲,夏华强..精液液化相关基因单核苷酸多态性研究[J].中国医学创新,2016,13(17):23-25,3.基金项目
江西省自然科学基金计划 （）
