中国医学创新2016，Vol.13Issue(18)：1-4,4.DOI:10.3969/j.issn.1674-4985.2016.18.001
豚鼠膀胱Cajal样间质细胞钙震荡特性
Calcium Oscillation Characteristics of Interstitial Cells of Cajal in Guinea Pig Bladder
摘要
Abstract
Objective:To ascertain the spontaneous and cyclical calcium oscillation in Cajal-like interstitial cells of Cajal in the bladder of adult guinea-pig.Method:The V-type enzyme collagenase was used to separate primary cells from myoblast of the preparation muscle tissue,primary cells were cultured.Using fluo-4 AM dye the Cells.The change of calcium fluorescence intensity was recorded with laser scanning microscope imaging. The changes of the intracellular calcium concentration was on behalf with the Fluorescence intensity.Result:The Cajal-like interstitial cells maintained its inherent characteristics by primary cultivation.We could see that the cell had a spindle cell body with two slender bipolars.In the Cajal-like interstitial cells of bladder:there were 2 different types of spontaneous calcium,small irregular calcium oscillation and large oscillation slow calcium oscillation.The second type of calcium oscillation was completely different from those of smooth muscle cells.Conclusion:We have confirmed the existence of the spontaneous calcium oscillation in the Cajal-like interstitial cells in the bladder of adult guinea-pig,that like the pacemaker cells of interstitial cells of Cajal in the digestive tract.Implying that part of the Cajal-like interstitial cells may be the pacemaker cells of contraction or activity of bladder.关键词
钙震荡/Cajal样间质细胞/膀胱/起搏细胞/激光共聚焦显微镜Key words
Calcium oscillation/Cajal-like cells/Bladder/Pacemaker cell/Laser scanning confocal microscopy引用本文复制引用
朱国栋,王勤章..豚鼠膀胱Cajal样间质细胞钙震荡特性[J].中国医学创新,2016,13(18):1-4,4.基金项目
国家自然科学基金 （）
