作物研究2016，Vol.30Issue(4)：347-352,6.DOI:10.16848/j.cnki.issn.1001-5280.2016.04.01
芥菜型油菜A9染色体BAC物理图谱构建及比较基因组分析
Construction of BAC Physical Map on Chro moso me A9 in Brassica juncea and Co mparative Geno me Analysis
摘要
Abstract
To abtain a precise physical map of Brassica juncea chromosome A9,the markers located on the A9 chromosome were used in this study to screen Brassica juncea BAC library by three-dimensional mixed PCR screening. A total of 3200 positive BAC were screened out;constructing a physical map composed of 16 contigs and 4 singletons. According to the principle of the minimum path,435 BACs were chosen as seed BACs to represent the whole physical map and were subject to end sequencing,at last,862 qualified sequences were received. As the average insert size of BAC is 126 kb,the contigs accounted up to 52. 08 M,covering 1. 37 times of chromosome A9. By BLAST,the seed BAC end sequences were mapped to chromosome A9 pseudomolecule of Brassica rapa and Brassica napus respectively,results showed that the physical map covered 36. 4 Mb and 31. 0 Mb,equivalent to 93. 9%、91. 8% of the whole genome assemble length. Among the 862 BAC end sequences,702 sequences (81. 4%)were located on Brassica rapa A9 chromosome and 646 sequences(74. 9%)to Brassica napus A9 chromosome,which means Brassica juncea A9 chromosome have higher homology with Brassica rapa A9 chromosome than Brassica napus A9 chromosome. Compared with Brassica napus A9 pseudomolecule and BAC end se-quence of Brassica juncea A9 chromosome,there are two chromosome inversions about 1. 4 Mb、3. 6 Mb in length at the po-sition of 6413031-7827177 and 14856710-18458808 in Brassica rapa A9. While compared with BAC end sequence of Bras-sica juncea A9,there is a inserted mutation about 0. 98 Mb in length at the position of 15676847-16064302 of Brassica na-pus A9 pseudomolecule,which lie in one of the two chromosome inversions and was consist with the predicted position of centromere of Brassica napus A9 chromosome remained to assembled.关键词
芥菜型油菜/A9染色体/物理图谱/比较基因组Key words
Brassica juncea/chromosome A9/physical map/compare genomics分类
农业科技引用本文复制引用
刘芳瑛,陆赢,王卓,刘显军,胡学芳,刘旭东,徐海鹏,刘忠松..芥菜型油菜A9染色体BAC物理图谱构建及比较基因组分析[J].作物研究,2016,30(4):347-352,6.基金项目
国家自然科学基金（30971799）。 （）
