作物研究2016，Vol.30Issue(4)：353-358,6.DOI:10.16848/j.cnki.issn.1001-5280.2016.04.02
芥菜型油菜B03染色体黄籽基因区域BAC重叠群的构建及分析
Construction and Analysis of BAC Contigs Around the Yellow-Seeded Locus of the Chro moso me B03 in Brassica juncea
摘要
Abstract
The yellow seed genes were located in 1. 5 cM region of B03 linkage group of Brassica juncea in previous stud-ies. Yellow seed genetic regions (through the mustard RNASeq technology of rape seed non redundant genes)and BESs tightly linked to yellow seed genes of B03 were used to design markers in this study,and the markers were used to screen Brassica juncea mapping population parents purple leaf mustard BAC library (ZBjuH BAC library). In this study,320 markers were exploited and 920 BACs screened out from them,483 BACs of which were subject to end sequencing,and 860 sequences were returned. Six contigs (about 3. 5 Mb)were constructed around yellow seed coat gene on B03 chromosome in B. juncea. It was found that highly repetitive sequences are present in the area with yellow seed B03,A03,and A09.关键词
芥菜型油菜/黄籽基因/B03染色体/BAC重叠群Key words
Brassica juncea/yellow seed coat gene/B03 chromosome/BAC contig分类
农业科技引用本文复制引用
徐海鹏,刘显军,陆赢,马鹏,胡庆一,刘忠松..芥菜型油菜B03染色体黄籽基因区域BAC重叠群的构建及分析[J].作物研究,2016,30(4):353-358,6.基金项目
国家自然科学基金（30471098）。 （）
