组织工程与重建外科杂志2016，Vol.12Issue(3)：160-162,3.DOI:10.3969/j.issn.1673-0364.2016.03.004
应用小室模型再生毛发的实验研究
The Research of Hair Regeneration by Chamber Assay
杨颖 1杨志岗 1肖苒1
作者信息
- 1. 100144 北京市 中国医学科学院整形外科医院研究中心
- 折叠
摘要
Abstract
Objective To establish a hair reconstitution chamber model for experimental study of hair regeneration. Methods Epidermal and dermal cells from newborn C57BL/6 mice were isolated, mixed and injected into the chambers. 7 days after the removal of the chambers, hair regeneration at the wound site was continually observed. At 30 days, the skin of wound sites was obtained and HE staining was performed to observe the morphological structures. Results Wound healing and hair regeneration were observed at chamber implantation site, the regenerated hair had no significant difference from normal skin, with well-maintained polarity and density. By analyzing the wound sites without hair regeneration, we found that chamber implantation sites have great influence on hair regeneration. Conclusion Newborn epidermal and dermal cells can successfully regenerate hair by chamber assay, chamber assay is a good animal model for hair regeneration, and chamber implantation sites play an important role in the success foundation of chamber model.关键词
毛发再生/毛囊重建/小室模型Key words
Hair regeneration/Hair follicle reconstitution/Chamber model分类
生物科学引用本文复制引用
杨颖,杨志岗,肖苒..应用小室模型再生毛发的实验研究[J].组织工程与重建外科杂志,2016,12(3):160-162,3.
