北京林业大学学报2016，Vol.38Issue(5)：67-76,10.DOI:10.13332/j.1000--1522.20150507
超表达杨树RPEase基因促进了拟南芥的生长发育摇
Over-expression of RPEase gene promotes the growth and development of Arabidopsis thaliana
摘要
Abstract
The Calvin cycle is an irreplaceable pathway for photosynthetic organisms to assimilate CO2 from the air and therefore plays a crucial role in plant growth and development. The ribulose-phosphate 3-epimerase (RPEase:EC 5. 1. 3. 1) is one of the integral enzymes in the Calvin cycle regeneration phase. In our study PdRPE gene was cloned from fast-growing hybrid poplar NE-19 ( Populus nigra í ( Populus deltoids í Populus nigra) ) , and then PdRPE was transformed into Arabidopsis thaliana by the floral dip method. Using PCR and Green Fluorescent Protein ( GFP ) detection methods, we obtained the CaMV35S:PdRPE:GFP transgenic plants. Then we validated the growth status and physiological indexes of wild type (Col-0), overexpressing (OxPdRPE), mutant (rpe) and complementary (rpe/PdRPE) lines. The results indicated that in overexpressing lines RPEase activity increased significantly ( P <0. 05). When well-watered, the overexpressing lines had lower leaf stomatal density, larger stomatal size, improved water use efficiency and higher net photosynthetic rate, showing a better growth condition and more starch accumulation. When exposed to 10 days of short-term drought, the net photosynthetic rate and water use efficiency of overexpressing lines were still significantly higher than those of the other three strains (P<0. 01). Therefore, we reach the conclusion that over-expression of RPEase gene can promote the plant growth and development and enhance the resistance of short-duration drought in Arabidopsis thaliana.关键词
杨树/卡尔文循环/RPEase/生长/发育/水分利用效率Key words
poplar/Calvin cycle/RPEase/growth/development/water use efficiency分类
农业科技引用本文复制引用
王丹,安轶,韩潇,周扬颜,王厚领,郭惠红,夏新莉,尹伟伦..超表达杨树RPEase基因促进了拟南芥的生长发育摇[J].北京林业大学学报,2016,38(5):67-76,10.基金项目
“十二五”国家科技支撑计划课题(2015BAD07B01)、国家自然科学基金项目(31270656)、北京市科学研究与研究生培养共建项目。 （2015BAD07B01）
