北京林业大学学报2016，Vol.38Issue(5)：77-83,7.DOI:10.13332/j.1000--1522.20150033
油松胚珠转录组微卫星特征分析
Characterization of microsatellites of ovule transcriptome in Pinus tabulaeformis
摘要
Abstract
In order to understand the molecular mechanism of regulating ovule development of Pinus tabulae-formis thoroughly and clarify the influence of microsatellites in ovule development, we used the program MISA to mine and analyze the high-throughput sequencing in earlier experiments to seek the microsatellites of transcriptome, in an attempt to provide significant information and data for deeper research. A total of 1 412 SSRs were identified in 1 274 unigenes with one SSR per 2. 15 kb. Among 87 SSR repeat motifs of ovule transcriptome, ( A/T ) n was in the maximum proportion, accounting for 45. 89%. Mononucleotide and trinucleotide repeats were the two more abundant in all repeat types, accounting for 46. 88% and 34. 99%, respectively. The microsatellites with length below 20 bp were in the majority and those over 20 bp amounted only 5. 67%. It showed a negative relation between the frequency of occurrences and the length of microsatellites. Most of SSRs were in untranslated region, and only 232 SSRs were located in protein-coding regions, while 18 in both of them. Trinucleotide repeats were the richest in protein-coding regions (105, 45. 26%).关键词
油松/胚珠/转录组/微卫星Key words
Pinus tabulaeformis/ovule/transcription/microsatellite分类
农业科技引用本文复制引用
安俊,郑彩霞,姚阳,陈彬丽..油松胚珠转录组微卫星特征分析[J].北京林业大学学报,2016,38(5):77-83,7.基金项目
国家自然科学基金项目(31170563)。 （31170563）
