北京中医药大学学报2016，Vol.39Issue(5)：383-389,7.DOI:10.3969/j.issn.1006-2157.2016.05.007
地椒抗氧化活性部位化学成分的研究∗
Antioxidant activities and chemical compositions of Thymus quinquecosta-tus Celak
摘要
Abstract
Objective To study the antioxidant activities and chemical compositions of Thymus quinque-costatus Celak ( dijiao ) . Methods The antioxidant activities of five polar fractions of dijiao were screened and evaluated using DPPH and ABTS free radical scavenging assays. After the chemical compo-sitions were separated and purified by using silica gel chromatography, Sephadex LH-20 chromatography and MCI GEL CHP-20P column chromatography, the chemical compositions were identified by MS and NMR spectrometers, and records of literature. Results From the EtOAc and n-BuOH fractions which showed high antioxidant activity, total 18 compositions were separated and identified: scutellarin (“1 ”short for peak No. 1, the same below), scutellarein (2), 5,6,7-trihydroxy-4′-methoxyflavone (3), xanthomicrol (4), apigenin (5), 4′-methoxyluteolin (6), luteolin-7-O-β-D-glucopyranoside (7), lute-olin (8),and rutin (9), danshensu (10), vanillic acid (11), protocatechuic acid (12), chlorogenic acid (13), caffeic acid (14), ferulic acid (15), 2,6-dihydroxy-4-isopropylphenyl-β-D-glucopyranoside(16), olenolic cid (17) and daucosterol (18). Conclusion The composition 1,2, 3 and 16 were the first reported in the genus Thymus, and 4~8, 10~12, 14~15 and 17~18 were found first in dijiao.关键词
地椒/抗氧化活性/野黄芩苷/野黄芩素/4′,5-二羟基-6 ,7 ,8 ,-三甲氧基黄酮Key words
Thymus quinquecostatus Celak/antioxidant activity/scutellarin/scutellarein/xanthomicrol分类
医药卫生引用本文复制引用
颜承,陈晓怡,隋宏,田玉欣,王炎,白少娟,赵怡程,石任兵,折改梅..地椒抗氧化活性部位化学成分的研究∗[J].北京中医药大学学报,2016,39(5):383-389,7.基金项目
北京中医药大学创新团队项目(No.2011-CXTD-12),陕西省农业公关项目(No. S2014NY2608),北京中医药大学自主课题项目(No.2014-JYBZZ-JS-026) （No.2011-CXTD-12）
