北京中医药大学学报2016，Vol.39Issue(5)：390-394,5.DOI:10.3969/j.issn.1006-2157.2016.05.008
金莲清热胶囊质量标准研究∗
Improving the quality standard for Jinlian Qingre Capsule
摘要
Abstract
Objective To improve the quality standard of Jinlian Qingre Capsule. Methods Chinese Globeflower Flower(Trollii Chinensis Flos, Jinlianhua),Dyers Woad Leaf(Folium Isatidis, Daqingye), Figwort Root ( Srophulariae Radix, Xuanshen ) and Bitter Apricot Seed ( Semen Armeniacae Amarum, Kuxingren) in Jinlian Qingre Capsule were identified using thin layer chromdograph ( TLC) . The content of vitexin was detected using HPLC with a Phenomenex Luna C18 (2)100A column(250 mm × 4. 6 mm,5μm), a mobile phase of acetonitrile- 0. 4% ammonium acetate(15∶85)at the flow rate of 1. 0 mL/min, detection wave length at 340 nm and the column temperature at 25 ℃. Results Main spots of Chinese Globeflower Flower,Dyers Woad Leaf,Figwort Root and Bitter Apricot Seed in TLC were clear and sepa-rated well. A good linearity was obtained for vitexin in the range of 0. 06~0. 57 μg(r=0. 999 9)with mean sample recovery rate of 99 . 7% and RSD of 2 . 1%. Conclusion Method of TLC and HPLC estab-lished in this study which appears to be highly specific,accurate and repeatable can be used in the quality control of the Jinlian Qingre Capsule.关键词
薄层色谱/高效液相色谱/金莲清热胶囊/牡荆苷/质量标准Key words
TLC/HPLC/Jinlian Qingre Capsule/vitexin/quality standard分类
医药卫生引用本文复制引用
丁锐,贺凯,张海旭,王庆..金莲清热胶囊质量标准研究∗[J].北京中医药大学学报,2016,39(5):390-394,5.基金项目
国家药典委员会标准提高项目 （）
