北京中医药大学学报2016，Vol.39Issue(5)：406-412,7.DOI:10.3969/j.issn.1006-2157.2016.05.011
艾灸“足三里”对胃黏膜损伤大鼠内源性保护因子含量及相关蛋白表达的影响
Effects of moxibustion at Zusanli(ST36) on endogenous protective factors and related proteins expression in gastric mucosal lesions rats after dama-ging nucleus tractus solitarii
摘要
Abstract
Objective To study the role of nucleus tractus solitarii ( NTS ) on the protective effects of moxibustion on gastric mucosa lesion in the nervous pathway by investigating the effects of moxibustion at"Zusanli" ( ST 36 ) on endogenous protective factors and gap-associated protein expression of gastric mu-cosal lesion rats after damaging their nucleus tractus solitarii ( NTS) . Methods 48 healthy SD rats, SPF grade, were divided into four groups using random number table ( each n=12 ): normal group, model group, moxibustion group, moxibustion + NTS damaged group. Three days after bilateral NTS damaged in moxibustion + NTS damaged group, gastric mucosal lesion rat model was induced by intragastrical ( i. g. ) administration of anhydrous ethanol at dose of 6 mL/kg and aspirin mixed suspension at dose of 200 mg/kg for consecutive three days. The next day, rats in moxibustion group and moxibustion + NTS damaged group were treated by moxibustion at Zusanli ( ST 36 ) twice daily for consecutive three days. All rats were sacrificed, then their serum and gastric tissue were collected. Ulcer index ( UI) was calcu-lated, and the expressions of epidermal growth factor ( EGF) , nitric oxide ( NO) , Bcl-2 anti-apoptotic proteins ( Bcl-2 ) and pro-apoptotic protein ( Bax ) were detected. Results Compared with normal group, UI in model group increased significantly(P <0. 05), decreased in moxibustion group(P <0. 05). Compared with model group, content of EGF and NO in serum and gastric tissue increased remark-ably in moxibustion group(P<0. 05)which were higher than those in moxibustion + NTS damaged group ( P<0 . 05 ) . Protein expression of Bcl-2 increased significantly in moxibustion group and moxibustion +NTS damage group compared with model group, while expression of Bax protein decreased with increasing of AI ( P<0 . 05 ) . Conclusion Treatment of moxibustion at Zusanli ( ST 36 ) can regulate the expres-sion of endogenous protective factors and proteins to protect rats’ gastric mucosa, which was affected by damaging NTS. It is suggested that NTS be the important regulatory location in the signaling pathway of protective effects of moxibustion at Zusanli on gastric mucosal.关键词
艾灸/足三里穴/孤束核损毁/胃黏膜损伤/大鼠Key words
moxibustion/damage nucleus tractus solitarii/gastric mucosal lesions/rats分类
医药卫生引用本文复制引用
向娟,彭亮,陈果,欧阳里知,李飞,向丽婷,陈英,于隽,杨舟,李铁浪..艾灸“足三里”对胃黏膜损伤大鼠内源性保护因子含量及相关蛋白表达的影响[J].北京中医药大学学报,2016,39(5):406-412,7.基金项目
国家自然科学基金资助项目(No.81303050),湖南省优秀博士论文资助项目(No.2014-2016) （No.81303050）
