草业学报2016，Vol.25Issue(5)：69-77,9.DOI:10.11686/cyxb2015502
老芒麦种子发育过程中生殖枝中糖分、丙酮酸含量及硝酸还原酶活性变化
Changes in sugar,pyruvic acid content and nitrate reductase activity of Elymus sibiricus reproductive branches during seed development
摘要
Abstract
When Elymus sibiricus was in the filling stage,milk stage and dough stage,respectively,compo-nents of reproductive branch,including leave,stem,spike-handle,spike-stalk and seed all were used as re-search subject.Some index,including soluble sugars (SS),sucrose (SU),starch (ST),respiration intermedi-ate product pyruvate acid (PA)content and nitrate reductase activity (NR)were measured.The objective of study was to analyze the difference of sugar content,respiration rate and metabolic rate of nitrogen during the seed development and among either component,to research the transfer rule of filling material from the source to the library during the seed development,and to explore the relationship between seed plumpness and materi-al content of components.The results showed that:1)SS,SU and PA content and NR activity exists high sig-nificant difference during the seed development and among either components (P <0.01 ).ST content exists significant difference among either component,and only a little change during the seed development.2)With seed developing,SS and SU content of either component significantly decline (P <0.05),but ST content has no significant rising.PA content significantly increases at milk stage,but significantly decreases at dough stage (P <0.05).NR activity in the filling stage and milk stage were no difference,but significantly decrease in the dough stage (P <0.05).3)During the seed development,PA content of leave is significantly higher than that of other components,but SS,SU and ST content and NR activity in the spike-stalk are several times higher than those of other components.4)During filling stage,NR activity has a significantly positive correlation with SS,ST,SU content and PA activity (P <0.05),with the correlation order of ST>SU>SS>PA.During milk stage,the correlation order between NR activity and sugar content was SS>ST>SU,and NR activity has no significant correlation with PA content (P <0.05).During dough stage,NR activity only has a significant correlation with SS and ST content (P <0.01).5)Seed plumpness has a significantly positive correlation with SS content of leave,PA content of spike-handle and NR activity and ST content of seed (P <0.05),and a sig-nificantly negative correlation with NR activity and SU content of spike-stalk (P <0.05).In a word,compo-nents of reproductive branch all participate in material transportation from source to library during seed devel-opment.At the milk stage,transportation speed and storage deposit are fastest,and spike-stalk has a control function on source-library flow.Nitrogen metabolism rate is significantly associated with seed plumpness.关键词
老芒麦/生殖枝/种子发育/糖分运输/呼吸强度/氮代谢Key words
Elymus sibiricus/reproductive branch/seed development/sugar transportation/respiration inten-sity/nitrogen metabolism引用本文复制引用
刘金平,范宣,游明鸿,王思思,宗人旭..老芒麦种子发育过程中生殖枝中糖分、丙酮酸含量及硝酸还原酶活性变化[J].草业学报,2016,25(5):69-77,9.基金项目
四川省科技厅应用基础项目（2012JY0062）和国家牧草产业体系阿坝试验站（CARS-35-35）项目资助。 （）
