草业学报2016，Vol.25Issue(5)：78-86,9.DOI:10.11686/cyxb2015366
印度梨形孢真菌对干旱胁迫下紫花苜蓿生长及抗旱性的影响
Effects of Piriformospora indica fungus on growth and drought resistance in alfalfa under water deficit stress
摘要
Abstract
Pot experiments were conducted to evaluate the growth and drought resistance of alfalfa (Medicago sativa )seedlings colonized by Piriformospora indica fungus.The experiment comprised a 2×2 factorial com-bination of inoculation (inoculated,I,or un-inoculated,NI)and drought (droughted,D or not,ND)treat-ments,giving 4 combinations:I-D,I-ND,NI-D (reference),and NI-ND.For the inoculated plants,40 mL of inoculant with P .indica mycelium was applied,and for control plants,40 mL of sterile water was added.Soil water content was 75% -80% of field capacity for the normal irrigation treatment and 15% -20% for the drought treatments.Fresh shoot and root weights,root length,dry shoot and root weights,chlorophyll <br> (SPAD),relative water content (RWC),antioxidase enzyme activity,O2 - ,methane dicarboxylic aldehyde (MDA)and osmolytes were determined for all treatments.The fungus strongly colonized the roots of alfalfa, infecting 87.4% of seedlings.When the colonized plants were exposed to drought stress,the fresh shoot weight,dry shoot weight,chlorophyll,relative water content and the number of leaves per plant were promo-ted significantly by 63.4%,69.2%,12.5%,17.1% and 5.7,respectively.In addition the fresh root weight, dry root weight,length of taproot and number of lateral roots were improved significantly by 33.3%,57.1%, 5.1 cm and 5 roots,respectively,compared to the reference plants (NI-D).Furthermore,the activities of su-per-oxide,peroxidases,catalases,and the levels of proline and of soluble sugar in the leaves increased by 1.71, 1.27,1.22,1.49 and 1.48 times,respectively,and the contents of O2 - and MDA decreased significantly.In summary,P .indica fungus enhanced the growth and drought tolerance of alfalfa in the seeding stage by stim-ulating the activity of antioxidant enzymes and the content of osmolytes,while under normal irrigation,the fungus had no significant effect on the growth of alfalfa.关键词
紫花苜蓿/苗期/印度梨形孢/促进生长/抗旱Key words
alfalfa (Medicago sativa)/seeding stage/Piriformospora indica/growth promotion/drought re-sistance引用本文复制引用
武美燕,蒿若超,张文英..印度梨形孢真菌对干旱胁迫下紫花苜蓿生长及抗旱性的影响[J].草业学报,2016,25(5):78-86,9.基金项目
国家公益性行业（农业）科研专项（201203032），长江大学作物抗逆技术研究中心开放基金（2014001）和湿地生态与农业利用教育部工程研究中心开放基金项目（KF201506）资助。 （）
