草业学报2016，Vol.25Issue(5)：87-94,8.DOI:10.11686/cyxb2016024
接种尖镰孢菌对枸杞苯丙烷代谢关键酶及产物的影响
Effects of key enzymes and products in phenylpropanoid pathway of Lycium infected by Fusarium oxysporum
摘要
Abstract
In this study,the phenylpropanoid pathway of the interaction of wolfberry and root rot pathogens will be discussed.The relationship between wolfberry phenylpropanoid pathway and disease resistance will be clarified.In this study,the experimental materials are cultivated varieties NingqiⅠ,NingqiⅡ;domestic wild species Lycium chinense ,L.barbarum;wild species of America L.brevipes ,L.exsertum.The strong patho-genic Fusarium oxysporum isolated from the onset of wolfberry will be inoculation by root cutting method. And the anti-sense wolfbeey will be choose.This 2 kinds of wolfberry as materials to study the change of phen-ylalanine ammonia lyase,cinnamic acid hydroxylase,4-hydroxylase p-coumaric acyl-coenzyme A ligase,phe-nolics and flavonoids in 20 days.The results show that the disease resistance of wild materials are strong than the cultivated material in 6 kinds of test materials which were inoculated with wolfberry root rot strong patho-gen F .oxysporum.Ningqi Ⅰis susceptible materials.The resistance to root rot disease of L.exsertum are strongest.The key enzymes of phenylpropanoid pathway like phenylalnine ammonialyase,trans-cinnamic acid- <br> 4-hydroxylase,4-coumarate:coenzyme A ligase activity are significantly higher than Ningqi Ⅰ.Initially identi-fied the phenylalnine ammonialyase,trans-cinnamic acid-4-hydroxylase,4-coumarate:coenzyme A ligase activ-ity and flavonoid content of phenylpropanoid pathway of test material resistance to root rot disease was posi-tively correlated.Therefore,the phenylalnine ammonialyase,trans-cinnamic acid-4-hydroxylase,4-coumarate:coenzyme A ligase enzyme activity and flavonoid content can be a screening of anti-Chinese wolfberry Fusarium root rot of biochemical indexes preliminary.关键词
枸杞/根腐病/尖镰孢菌/苯丙烷代谢/抗病性Key words
Lycium spp./root rot/Fusarium oxysporum/phenylpropanoid pathway/disease resistance引用本文复制引用
李捷,冯丽丹,杨成德,王有科,何静,张宝琳,陈秀蓉..接种尖镰孢菌对枸杞苯丙烷代谢关键酶及产物的影响[J].草业学报,2016,25(5):87-94,8.基金项目
国家林业局“948”项目（2011-4-39），甘肃中草药科技攻关项目（GYC11-01），甘肃省科技支撑项目（1304FKCA054）和甘肃农业大学盛彤笙科技创新基金（GSAU-STS-1338）资助。 （）
