草业学报2016,Vol.25Issue(5):125-133,9.DOI:10.11686/cyxb2015336
利用 SSR 标记分析高粱属种质资源的遗传多样性
Analysis of genetic diversity in Sorghum germplasm collections using SSR markers
摘要
Abstract
Fifteen SSR markers were used to quantify the genetic diversity of the core germplasm collections of sorghum.A total of 161 accessions were assayed.118 bands were generated,among which 88 (75.21%)were polymorphic.The average value of polymorphic information content (PIC)was 0.699 (0.612-0.806);the av-erage Nei’s gene diversity index (H)was 0.696;and the average Shannon information index (I)was 1.393. The genetic similarities among the 161 accessions ranged from 0.636 to 0.977.The UPGMA dendrogram indi-cated that the 161 Sorghum germplasm collections were clustered into three groups with a threshold of 0.682, and that the 129 Sorghum sudanense and Sorghum bicolorx ×Sorghum sudanense germplasm resources were di-vided into two clusters at a threshold of 0.671.This study demonstrated that there is considerable genetic di-versity conserved in Sorghum germplasm collections and SSR is an ideal molecular marker for assessing this di-versity.关键词
高粱属/种质资源/SSR/遗传多样性Key words
Sorghum/germplasm resource/simple sequence repeat (SSR)/genetic diversity引用本文复制引用
王芳,高秋,王杰,马金星,孙娟..利用 SSR 标记分析高粱属种质资源的遗传多样性[J].草业学报,2016,25(5):125-133,9.基金项目
国家牧草产业技术体系项目资助。 ()