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利用 SSR 标记分析高粱属种质资源的遗传多样性

王芳 高秋 王杰 马金星 孙娟

草业学报2016,Vol.25Issue(5):125-133,9.
草业学报2016,Vol.25Issue(5):125-133,9.DOI:10.11686/cyxb2015336

利用 SSR 标记分析高粱属种质资源的遗传多样性

Analysis of genetic diversity in Sorghum germplasm collections using SSR markers

王芳 1高秋 2王杰 1马金星 2孙娟1

作者信息

  • 1. 青岛农业大学经济草本植物应用研究所,山东 青岛 266109
  • 2. 农业部全国草产品质量监督检验测试中心,北京 100125
  • 折叠

摘要

Abstract

Fifteen SSR markers were used to quantify the genetic diversity of the core germplasm collections of sorghum.A total of 161 accessions were assayed.118 bands were generated,among which 88 (75.21%)were polymorphic.The average value of polymorphic information content (PIC)was 0.699 (0.612-0.806);the av-erage Nei’s gene diversity index (H)was 0.696;and the average Shannon information index (I)was 1.393. The genetic similarities among the 161 accessions ranged from 0.636 to 0.977.The UPGMA dendrogram indi-cated that the 161 Sorghum germplasm collections were clustered into three groups with a threshold of 0.682, and that the 129 Sorghum sudanense and Sorghum bicolorx ×Sorghum sudanense germplasm resources were di-vided into two clusters at a threshold of 0.671.This study demonstrated that there is considerable genetic di-versity conserved in Sorghum germplasm collections and SSR is an ideal molecular marker for assessing this di-versity.

关键词

高粱属/种质资源/SSR/遗传多样性

Key words

Sorghum/germplasm resource/simple sequence repeat (SSR)/genetic diversity

引用本文复制引用

王芳,高秋,王杰,马金星,孙娟..利用 SSR 标记分析高粱属种质资源的遗传多样性[J].草业学报,2016,25(5):125-133,9.

基金项目

国家牧草产业技术体系项目资助。 ()

草业学报

OA北大核心CSCDCSTPCD

1004-5759

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