长春中医药大学学报2016，Vol.32Issue(3)：451-454,4.DOI:10.13463/j.cnki.cczyy.2016.03.005
黄芪多糖对阿尔茨海默病大鼠海马超微结构和低密度脂蛋白受体相关蛋白1水平的影响
Astragalus polysaccharides on ultrastructure and lipoprotein receptor-related protein 1 of hippocampus in Alzheimerˊs disease rat
摘要
Abstract
Objective Effects of astragalus polysaccharides(APS)on ultrastructure and lipoprotein receptor-re-lated protein 1(LRP1)of hippocampus in Alzheimerˊs disease(AD)rat. Methods Wistar rat were randomly divided into control group,model group,treatment group(Piracetam 0. 5g / kg),APS high-dose group(APS 0. 8 g / kg),APS medium-dose group(APS 0. 4 g / kg),APS low-dose group(APS 0. 2 g / kg). Aβ1-42 was injected into the bilateral hip-pocampus of rat to induce AD models. The rat were killed after 30 days continuous treating. After the treatment all ani-mals were sacrificed,ultrastructure of hippocampus was observed,calculated the organ coefficients of splenic and thy-mus. The levels of Aβ1-40 and LRP1 in the hippocampus were measured by enzyme-linked immunosorbent assay (ELISA). The levels of Aβ1-40 in the serum were measured by ELISA. The content of LRP1 mRNA in the hippocampus was determined by reverse-transcription PCR(RT-PCR). Results Compared with the model group,the APS high-dose group and APS medial-dose group brain maintain normal morphological structure of hippocampus was significantly increased,the organ coefficients of splenic and thymus significantly increased(P < 0. 05),the levels of Aβ1-40 in the hippocampus were significantly decreased(P < 0. 05),the levels of LRP1 in the hippocampus and the levels of Aβ1-40 in the serum were significantly increased(P < 0. 05). Conclusion APS can maintain normal morphological structure of hippocampus of AD rat,increased LRP1 responses in the hippocampus tissue,organ coefficients of splenic and thy-mus.关键词
黄芪多糖/阿尔茨海默病/超微结构/LRP1Key words
Astragalus polysaccharides/Alzheimerˊs disease/ultrastructure/LRP1分类
医药卫生引用本文复制引用
姜波,费洪新,宋磊,尹丽颖,仲丽丽,张英博,李宝龙,周忠光..黄芪多糖对阿尔茨海默病大鼠海马超微结构和低密度脂蛋白受体相关蛋白1水平的影响[J].长春中医药大学学报,2016,32(3):451-454,4.基金项目
国家自然科学基金（81373777，81173599）；高校博士学科点科研基金（博导类20132327110010）；黑龙江中医药大学博士后基金（LBH-Z14196）；黑龙江省青年基金（QC2015101）。 （）
