长春中医药大学学报2016，Vol.32Issue(3)：458-461,4.DOI:10.13463/j.cnki.cczyy.2016.03.007
以基因芯片探究针刺泻法对应激性高血压前期大鼠肾脏基因表达的影响
Twirling-reducing needling manipulation on gene expression in kidney of stress-induced pre-hypertension rats
摘要
Abstract
Objective To study the effect of twirling-reducing needling manipulation(TRNM)on gene expression in kidney of stress-induced pre-hypertension rat by gene chip technology. Methods Twenty-seven,male Wistar rats were randomly divided into 3 groups( n = 9 / group):blank control group,model control group and model + TRNM group. Foot-shocks combined with generated noise was applied in the preparation of stress-induced pre-hypertension model and lasted for 14 days. Twirling-reducing needling manipulation was used on Taichong(LR3)and Quchi(LI11) of TRNM group along the molding cycle. The model control and blank control group rats were grabbed in the same re-strainers for 20min without any other intervene. Systolic blood pressure(SBP)was tested one day before work and on the 3 rd,5 th,7 th,9 th,11 th,13 th and 15 th day. Gene expression profiles was determined by Rat Gene 2. 0 Array technology. Results SBP of blank control group rats stayed normal along the modeling cycle. At the 3rd day,SPB of model control group elevated to 120 mmHg(P < 0. 01),and continuously rose to 139 mmHg,along with changes like irritation,squealing,yellow urination and bloodshot eyes,which indicates the success of modeling. SBP of TRNM group was significantly lower(P < 0. 01)than model control group since the 5 th day. Microarray analysis showed that com-pared with model control group,106 genes were upregulated and 92 genes were downregulated in TRNM group. The expression of CYP1A1 and IGFBP-1,which have a close relationship with hypertension and organ damage,was signifi-cantly changed by Twirling-reducing needling. Conclusion Twirling-reducing needling manipulation at Taichong and Quchi can significantly lower the SBP of stress - induced pre-hypertension rats and effect its gene expression in kidney.关键词
针灸疗法/泻法/应激性高血压前期/基因芯片Key words
acupuncture/twirling-reducing needling manipulation/stress-induced pre-hypertension/gene chip分类
医药卫生引用本文复制引用
纪智,郭文,朱华超,谢晓佳,孙启胜,刘清国..以基因芯片探究针刺泻法对应激性高血压前期大鼠肾脏基因表达的影响[J].长春中医药大学学报,2016,32(3):458-461,4.基金项目
国家自然科学基金“基于系统生物学的针刺对应激性前期高血压的调控机制与干预靶点的研究”（81373727）。 （）
