长春中医药大学学报2016，Vol.32Issue(3)：462-463,464,3.DOI:10.13463/j.cnki.cczyy.2016.03.008
siRNA 沉默胸腺素β4对鹿茸间充质干细胞增殖的影响
Effect of siRNA-mediated thymosin β4 silence on proliferation in mesenchymal stem cell
摘要
Abstract
Objective To investigate the effects of siRNA-mediated thymosin β4(Thymosin β4 ,Tβ4 )silence on proliferation in activated mesenchymal stem cell(mesenchymal stem cell,MSC)and discuss the possible mechanism. Methods MSC cells were divided into 2 groups:negative control group and siRNA-Tβ4 transfection group. The siR-NA-Tβ4 was transfected into MSC cells and cell proliferation was measured by MTT,Real-time PCR detection ILK, AKT and CyclinD1mRNA expression quantity change,Western blot detection AKT and P-AKT protein content chan-ges. Results MSC proliferation was significantly inhibited in siRNA-Tβ4 transfection group(P < 0. 01);Expression of the ILK,CyclinD1 mRNA of MSC transfected with siRNA-Tβ4 is reduced,AKT quantity remains the same( P <0. 01);The siRNA-Tβ4 transfection group no change in total AKT protein,P-AKT protein content decreased. Conclu-sion The siRNA-mediated Tβ4 silence significantly inhibits MSC proliferation by up regulating the ILK,p-AKT and CyclinD1expression in MSC.关键词
RNA 干扰/Tβ4/间充质干细胞/ILK/ AKT 信号通路Key words
RNA interference/Tβ4/mesenchymal stem cell/ILK/ AKT signaling pathway分类
医药卫生引用本文复制引用
谢红梅,孟祥宇,孙天霞,张丽轩,赵雨,曲毅..siRNA 沉默胸腺素β4对鹿茸间充质干细胞增殖的影响[J].长春中医药大学学报,2016,32(3):462-463,464,3.基金项目
吉林省科技发展计划项目（20140622003JC）；吉林省科技发展计划项目（20130101142JC）。 （）
