长春中医药大学学报2016，Vol.32Issue(3)：464-466,3.DOI:10.13463/j.cnki.cczyy.2016.03.009
人参亲环素蛋白原核表达及其抗真菌活性
Prokaryotic expression of ginseng cyclophilin and the detected of antifungal activity
摘要
Abstract
Objective We investigated the antifungal activity of panax ginseng cyclophilin(pgCyP) in vitro.Methods In this study,we amplified pgCyP gene by RT-PCR,constructed prokaryotic expression plasmid and trans-formed into E. Coli. Target protein was induced by IPTG and affinity purified. Disc diffusion method was used to verify the antifungal activity of target protein. Results The full length of pgCyP gene is 522 bp,which codes for a protein 174 amino acids in length. After purified,the target protein showed a single band in SDS-PAGE. Antifungal test showed that the recombinant protein can obviously inhibit the growth of the Phytophthora cactorum. Conclusion pgC-yP was successfully cloned,expressed and antifungal activity characterization,which provides foundation for further re-search of pgCyP antifungal mechanism.关键词
亲环素/人参/原核表达/纯化/抗真菌活性Key words
Cyclophilin/Panax ginseng/prokaryotic expression/purification/antifungal activity分类
医药卫生引用本文复制引用
李帅军,才可新,张楠楠,孙天霞,赵雨,曲毅..人参亲环素蛋白原核表达及其抗真菌活性[J].长春中医药大学学报,2016,32(3):464-466,3.基金项目
吉林省经济结构战略调整引导资金专项项目（2014N155）。 （）
