分析化学2016，Vol.44Issue(6)：857-863,7.DOI:10.11895/j.issn.0253-3820.150934
基于1HNMR给药赭石后大鼠尿液的代谢组学分析
1 H NMR-Based Metabonomic Study on Urine from Haematitum-Treated Rats
摘要
Abstract
Wistar rats were intragastrically administered with different doses (2, 5 and 10 g / kg body weight) of haematitum. 1H NMR-based metabonomic analysis coupled with multivariate statistical analysis (principal component analysis and partial least squares-discriminant analysis) was used to analyze the metabolic profiles of the urine samples collected from the treated rats. Univariate analysis on the 1H NMR spectra of urine (1 d before administration, 1-5 d post administration) was used to screen out the potential features of haematitum. Significant treatment related changes were observed for the levels of citrate, tuarine, creatinine,α-ketoglutarate, succinate and dimethylglycine, which could be used as potential features of haematitum. A trend of recovery in connection with dose levels was observed overtime. Such biochemical changes indicated that haematitum treatment at the dose of 2, 5 and 10 g / kg body weight affected the Krebs cycle and glucose metabolism, energy metabolism, choline metabolism and dimethylglycine metabolism in rats. These changes may attribute to the disturbances of hepatic function in 10 g / kg body weight group.关键词
核磁共振/代谢组学/主成分分析/偏最小二乘判别分析/赭石Key words
Nuclear magnetic resonance/Metabonomics/Principal component analysis/Partial least squares-discriminant analysis/Haematitum引用本文复制引用
魏莹,万传玲,薛蓉,李晓晶,章文军,裴奉奎..基于1HNMR给药赭石后大鼠尿液的代谢组学分析[J].分析化学,2016,44(6):857-863,7.基金项目
本文系国家自然科学基金项目(Nos.20975097,21305134)资助This work was supported by the National Natural Science Foundation of China (Nos.20975097,21305134) （Nos.20975097,21305134）
