化工学报2016，Vol.5Issue(5)：2015-2021,7.DOI:10.11949/j.issn.0438-1157.20151432
多拷贝毕赤酵母表达猪胰岛素前体的动力学模型
Kinetic modelling of porcine insulin precursor (PIP) expressed by multi-copy recombinantPichia pastoris
摘要
Abstract
Kinetic modelling of recombinantPichia pastoris harboring multiple porcine insulin precursor (PIP) gene dosages was studied when the cells were grown in the fed-batch culture. The key parameters of this kinetics were estimated, including specific cell growth rate (h?1), PIP production rate (g·g?1·h?1) and substrate consumption rate (g·g?1·h?1) with nonlinear curve fit by Origin8.0. The results showed that both growth-associated production coefficient (α) and growth-associated metabolism coefficient (k1) increased with increasing copy numbers. The expression level of PIP reached the highest at the copy number of 12. These results suggested that rapid growth and lower metabolic burden of a high copy number effectively improved the production rate of target proteins. Furthermore, the predicted values based on the established kinetic model were in good agreement with the experimental data, indicating that the kinetic model could be used to describe recombinant PIP production process in fed-batch fermentation mode.关键词
重组毕赤酵母/猪胰岛素前体表达/拷贝数/动力学模型Key words
recombinantPichia pastoris/porcine insulin precursor expression/gene dosage/kinetic model分类
化学化工引用本文复制引用
陈丽,王玥,郭美锦,储炬,庄英萍..多拷贝毕赤酵母表达猪胰岛素前体的动力学模型[J].化工学报,2016,5(5):2015-2021,7.基金项目
农业公益性行业科研专项经费项目(201303046) （201303046）
国家高技术研究发展计划重点项目(2012AA021201) （2012AA021201）
生物反应器工程国重室开放课题资助(2060204).supported by theSpecial Fund for Agro-scientific Research in the Public Interest(201303046), the National High Technology Research and Development Program of China (2012AA021201), and the Open Project Program of State Key Laboratory of Bioreactor Engineering (2060204). （2060204）
