化工学报2016，Vol.5Issue(5)：2084-2092,9.DOI:10.11949/j.issn.0438-1157.20151570
玉米秸秆中不同木质素脱除方法对纤维素酶吸附及酶解效果的比较
Comparison of different lignin removal processes for corn stover on cellulase adsorption and enzymatic hydrolysis
摘要
Abstract
Experiments were conducted for various pretreated substrates to investigate the impact of lignin content on cellulase adsorption and substrate digestibility. Compared with other treatments, 2% (mass) NaOH pretreated solids with the highest level of lignin removal (85%) exhibited the highest accessibility to cellulase [4.7 mg protein·(gglucan)?1] and enzymatic digestibility (18.9%). The obtained maximum adsorption capacity (Wmax) and equilibrium constant (K) derived from fitting the Langmuir adsorption isotherm for different delignified substrates indicated that the removal of lignin benefited cellulase adsorption. The relationship between cellulase adsorption capacities and enzymatic digestibility for raw and pretreated solids correlated well (R2>0.8), supporting the hypothesis that carbohydrate conversion was primarily dominated by enhancing substrate accessibility owing to lignin removal. Nevertheless, further delignification by NaOH with concentrations of 3% (mass) and 4% (mass) was unfavorable to improving substrate accessibility to cellulase and enhancing carbohydrates conversion to monosaccharide. It appeared that the appropriate delignification to some degree was a significant pretreatment factor to be taken into consideration to achieve more effectively enzymatic digestibility.关键词
玉米秸秆/生物质/生物能源/纤维素酶吸附/预处理/木质素脱除/酶水解效率Key words
corn stover/biomass/bioenergy/cellulase adsorption/pretreatment/lignin removal/enzymatic digestibility分类
轻工纺织引用本文复制引用
田顺风,程力,顾正彪,洪雁,李兆丰,李才明..玉米秸秆中不同木质素脱除方法对纤维素酶吸附及酶解效果的比较[J].化工学报,2016,5(5):2084-2092,9.基金项目
国家自然科学基金重点项目(31230057) （31230057）
国家自然科学基金项目(31371787) （31371787）
十二五科技部国家重点科技研究与发展规划项目(2012BAD34B07).supported by the Key Program of the National Natural Science Foundation of China (31230057), the National Natural Science Foundation of China (31371787) and the Twelfth Five-Year National Key Technology Research and Development Program of the Ministry of Science and Technology of China (2012BAD34B07). （2012BAD34B07）
