生物钟基因Per1对人口腔鳞状细胞癌细胞增殖、凋亡、细胞周期和体内成瘤的影响及机理OA北大核心CSCDCSTPCD
Effects and mechanism of the circadian clock gene Per1 on the proliferation, apoptosis, cycle, and tumorigenicityin vivo of human oral squamous cell carcinoma
目的：探讨生物钟基因Per1对细胞周期相关基因的调控作用，以及对人口腔鳞状细胞癌细胞SCC15增殖、凋亡、细胞周期和体内成瘤的影响。方法构建3组针对Per1 RNA的短发夹RNA（shRNA）慢病毒重组质粒，转染SCC15细胞，用蛋白质印迹法（Western blot）及实时荧光定量PCR（qRT-PCR）检测筛选RNA干扰作用最强组为实验组，对照组（Control-shRNA）为对任何基因均无干扰效应的shRNA序列的质粒，空白组为未做任何处理…查看全部>>
Objective To determine the regulatory effects of the circadian clock gene Per1 on cell cycle-related genes and its influence on the proliferation, apoptosis, cycle, and tumorigenicity in vivo of human oral squamous cell carcinoma SCC15 cells. Methods Three groups of the short hairpin RNA (shRNA) of lentivirus recombinant plasmids were designed against the RNA of Per1 and then transfected to the SCC15 cells. The optimum interference group was screened through…查看全部>>
付小娟;杨凯;李晗雪;赵钦;陈丹
重庆医科大学附属第一医院口腔颌面外科，重庆 400016重庆医科大学附属第一医院口腔颌面外科，重庆 400016重庆医科大学附属第一医院口腔颌面外科，重庆 400016重庆医科大学附属第一医院口腔颌面外科，重庆 400016重庆医科大学附属第一医院口腔颌面外科，重庆 400016
医药卫生
生物钟Per1细胞周期基因口腔癌
circadian clockPer1cell cyclegeneoral carcinoma
《华西口腔医学杂志》 2016 (3)
255-261,7
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