华西口腔医学杂志2016，Vol.34Issue(3)：286-290,5.DOI:10.7518/hxkq.2016.03.014
降钙素基因相关肽通过Hippo通路调控小鼠骨髓间充质干细胞成骨分化的实验研究
Calcitonin gene-related peptide-induced osteogenic differentiation of mouse bone marrow stromal cells through Hippo pathwayin vitro
摘要
Abstract
Objective Previous studies have clarified that calcitonin gene-related peptide (CGRP) can promote the biological activity of osteoblasts. To further reveal the role of CGRP in bone repair, we studied its influence on osteogenic differentiation of mouse bone marrow stromal cells (BMSCs) and initially explored the effect of the Hippo signaling pathway with this process. Methods BMSCs were induced to osteogenic differentiate osteoblasts by different concentrations of CGRP for a screening of the optimal concentration. CGRP was added in BMSCs, then the activity of alkaline phosphatase (ALP) and the number of mineralized nodules were examined by specific ALP kits after 48 hours and alizarin red staining fluid after 7 days, respectively. The protein expression of p-Mst1/2 was measured by Western blot. Verteporfin was used to block the downstream Yap signaling. The mRNA expression of collagen typeⅠ(ColⅠ) and runt-related transcription factor 2 (Runx2) were detected by reverse transcription-polymerase chain reaction. Results Compared to the blank group, different concentrations of CGRP (10−9, 10-8, 10−7 mol·L-1), especially 10−8 mol·L-1, significantly increased the ALP activity of BMSCs (P<0.05). Alizarin red staining also showed more mineralized nodules in 10−8 mol·L-1 group. The expression of p-Mst1/2 increased in the CGRP group (P<0.05). Verteporfin treatment effectively decreased the mRNA expression of Runx2 and ColⅠ(P<0.05). Conclusion The Hippo signaling pathway plays a role in CGRP-induced osteogenic differentiation in mouse BMSCs.关键词
降钙素基因相关肽/骨髓间充质干细胞/Hippo信号通路Key words
calcitonin gene-related peptide/bone marrow stromal cells/Hippo signaling pathway分类
生物科学引用本文复制引用
王飞,张慧宇,窦予昕,李适廷,张纲,谭颖徽..降钙素基因相关肽通过Hippo通路调控小鼠骨髓间充质干细胞成骨分化的实验研究[J].华西口腔医学杂志,2016,34(3):286-290,5.基金项目
国家自然科学基金（81371110，81300873）Supported by：The National Natural Science Foundation of China(81371110,81300873) （81371110,81300873）
