华西口腔医学杂志2016，Vol.34Issue(3)：295-301,7.DOI:10.7518/hxkq.2016.03.016
针对变异链球菌的人源特异性靶向抗菌肽C16LL-37的生物学特性
Biological characteristics of a human specifically targeted antimicrobial peptide C16LL-37 againstStreptococcus mutans
摘要
Abstract
Objective This study aimed to evaluate the biological characteristics of a human specifically targeted antimicrobial peptide C16LL-37 against Streptococcus mutans (S. mutans). Methods In this study, an antimicrobial peptide LL-37, a peptide derived from CSPC16 (S. mutans competence stimulating peptide), and recombinant peptide C16LL-37 were synthesized by Fmoc-chemistry-based strategy. The selectivity and antibacterial activity of C16LL-37 were identified by the colony counting method on microbial culture plates. After treatment of C16LL-37 at 32 μmol·L−1, the morphological changes in S. mutans were observed by using scanning electron microscopy (SEM). In addition, enzyme-linked immunosorbent assay was used to evaluate the hemolytic activity and antibacterial activity of C16LL-37 under different conditions. Results 1) The minimum inhibitory concentration of C16LL-37 was 16 μmol·L−1, and the minimum bactericidal concentration was 64 μmol·L−1. 2) The survival rate of S. mutans was 3.46% after C16LL-37 treatment at 64 μmol·L−1 for 30 min, whereas it was 0% at 64 μmol·L−1 for 60 min. The survival rates of four other kinds of bacteria were more than 60% at any time (P<0.05). 3) The morphological change in S. mutans was observed after C16LL-37 treatment at 32 μmol·L−1 by using SEM. S. mutans presented an irregular shape, rough surface, and evident splitting. 4) The hemolysis rate of C16LL-37 (≤64 μmol·L−1) was less than 0.33%. 5) This study showed no significant influence on the antibacterial activity of C16LL-37 under different conditions, such as temperature, pH, salinity, and trypsin at low concentration (P>0.05). Conclusion C16LL-37 exhibited obvious specificity for S. mutans, strong antibacterial activity, low toxicity, and high stability. Thus, C16LL-37 has good potential in caries research and clinical application.关键词
变异链球菌/感受态刺激肽/抗菌肽/特异性靶向抗菌肽Key words
Streptococcus mutans/competence stimulating peptide/antimicrobial peptide/specifically targeted anti-microbial peptide分类
医药卫生引用本文复制引用
车春晓,姜科宇,马媛媛,曾飒,周建业,李志强,何祥一..针对变异链球菌的人源特异性靶向抗菌肽C16LL-37的生物学特性[J].华西口腔医学杂志,2016,34(3):295-301,7.基金项目
国家自然科学基金地区科学基金（31160124/C0309）；甘肃省自然科学基金（1204FKCA16，1308RJZA248）Supported by：Fund for Less Developed Regions of Natural Science Foundation of China (31160124/C0309) （31160124/C0309）
Natural Science Foundation of Gansu Province (1204FKCA16,1308RJZA248) （1204FKCA16,1308RJZA248）
