集美大学学报（自然科学版）2016，Vol.21Issue(3)：184-190,7.
褐藻胶裂解酶发酵工艺优化及其中试放大
Optimization and Scale-up of Alginate Lyase Fermentation Processes
摘要
Abstract
Microbulbifer sp�ALW1 isolate was subjected to fermentation parameters optimization in a⁃ttempt to increase the alginate lyase producing ability in a 5 L bioreactor. The results showed that the addition of 1 g/L alginate was better for enzyme production than double or half the concentration, and 25 ℃ was more conductive to enzyme accumulation than 20 ℃. In addition, when pH was controlled at 7�5, the peak of al⁃ginate lyase yield occurred earlier than that at natural pH, but the yields showed little change. Based on the optimized conditions, the fermentation processes for alginate lyase production were scaled up and the maxi⁃mum alginate lyase activities of 57�0 U/mL, 43�5 U/mL, and 38�3 U/mL were achieved in 20 L bioreactor, 200 L bioreactor and 500 L bioreactor respectively, which were 39�6%, 30�2% and 26�5% of the small scale level.关键词
产微球茎菌ALW1/褐藻胶裂解酶/发酵工艺/优化/中试放大Key words
Microbulbifer sp�ALW1/alginate lyase/fermentation process/optimization/scale-up of fermentation process分类
化学化工引用本文复制引用
陈艳红,杨帆,肖安风,倪辉,朱艳冰,蔡慧农..褐藻胶裂解酶发酵工艺优化及其中试放大[J].集美大学学报（自然科学版）,2016,21(3):184-190,7.基金项目
国家自然科学基金资助项目（31401632）；厦门市科技计划项目（3502Z20120005）；厦门南方海洋研究中心项目 （）
