寄生虫与医学昆虫学报2016，Vol.23Issue(1)：51-56,6.DOI:10.3969/j.issn.1005-0507.2016.01.009
家蝇幼虫抗菌肽对肝癌HepG2细胞增殖、侵袭及迁移的影响∗
EFFECT OF MUSCA DOMESTICA ANTIBACTERIAL PEPTIDES ON THE PROLIFERATION, INVASION AND MIGRATION OF HEPG2 CELLS
摘要
Abstract
In order to study the antitumor function of the housefly antibacterial peptide in a more comprehensive way, this article studied the antitumor function of housefly antimicrobial peptides on the tumor cell proliferation, invasion and migration. To induce antibacterial peptides, we use the needle to prick housefly then go through a process of low temperature centrifugal, solid phase extraction and freeze⁃drying to obtain antibacterial peptides crude extract, and then adjust antibacterial peptides to different concentrations to inhibit CCK8, cell migration and invasion experiment. The experiment show that housefly antimicrobial peptide can inhibit HepG2 cells proliferation, and the dose⁃response characteristics is closely effected by its concentration. Low concentration of antibacterial peptides (60μg/mL) can also effectively inhibit the migration and invasion of HepG2 cells. In conclusion, the crude extractings of antibacterial peptides from housefly larvae can inhibit the proliferation, invasion and migration of HepG2 cells.关键词
家蝇幼虫/抗菌肽/肝癌HepG2细胞/增殖/侵袭/迁移Key words
Crude extracting/Musca domestica/HepG2 cells/Antibacterial peptides/Proliferation/Invasion/Migration引用本文复制引用
邢佳欣,刘娟娟,聂守民,郭姗,赵瑞君,程璟侠..家蝇幼虫抗菌肽对肝癌HepG2细胞增殖、侵袭及迁移的影响∗[J].寄生虫与医学昆虫学报,2016,23(1):51-56,6.基金项目
山西省基础研究计划（自然科学基金）项目（2013011060-4）；太原市科学技术发展计划项目 （）
