江苏大学学报（医学版）2016，Vol.26Issue(3)：219-222,226,5.DOI:10.13312/j.issn.1671-7783.y150277
氯膦酸二钠脂质体对重症急性胰腺炎大鼠脑损伤的保护作用
Protective effects of clodronate-liposome on cerebral damage induced by severe acute pancreatitis in rats
摘要
Abstract
Objective:To investigate the protective effects of clodronate-liposome on cerebral damage in-duced by severe acute pancreatitis(SAP)in rats.Methods:Clodronate-liposome was prepared by means of thin film.The models of SAP were performed by prinking injection of 5%sodium taurocholate (2 mL/kg) into the pancreatic capsule.A total of 48 male healthy SD rats were randomly divided into the sham group (Sham),SAP+liposome group (LB),SAP+clodronate-liposome group (LC).LB group were injected with liposome (4 mL/kg)while LC group were injected with clodronate-liposomes (4 mL/kg)in tail vein immediately after the SAP model performed.The change of amylase (AMS)in serum and the level of IL-6, TNF-αwere obtained at 2 h and 6 h after operation,respectively.Pathologic alteration of pancreas was ob-served and pathological score of pancreatic tissue was also estimated.The water and Evan′s blue content of brain tissues,the content of myelin basic protein (MBP)in cerebrospinal fluid were detected.Results:Compared with Sham group,the serum levels of AMS,IL-6,TNF-αand pancreatic tissue pathological score were significant elevated at 2 and 6 hours in LB group(P<0.01).In LC group,the serum levels of AMS, IL-6,TNF-αand pancreatic tissue pathological score were significant decreased at 2 and 6 hours compared with LB group(P<0.01).Compared with Sham group,the water and Evan′s blue content of brain tissues, the content of MBP in cerebrospinal fluid were significant elevated at 2 and 6 hours in LB group (P <0.01 ).In LC group,the water and Evan′s blue content of brain tissues,the content of MBP in cerebrospi-nal fluid were significant decreased at 2 and 6 hours compared with LB group(P<0.01 ).Conclusion:Monocytes/macrophages can be depleted by phagocytosis of liposome encapsulated clodronate,which may contribute to protection of clodronate-liposome against cerebral damage caused by SAP in rats.关键词
重症急性胰腺炎/脑损伤/氯膦酸二钠脂质体/巨噬细胞Key words
severe acute pancreatitis/cerebral damage/clodronate-liposome/macrophages分类
医药卫生引用本文复制引用
刘源,李涛,党胜春,张建新,顾敏..氯膦酸二钠脂质体对重症急性胰腺炎大鼠脑损伤的保护作用[J].江苏大学学报（医学版）,2016,26(3):219-222,226,5.基金项目
江苏省自然科学基金资助项目（BK2012704）；镇江市社会发展项目 （）
