江苏大学学报（医学版）2016，Vol.26Issue(3)：227-230,4.DOI:10.13312/j.issn.1671-7783.y160005
小鼠主动脉环血管新生实验模型的优化
A modification of the mouse aortic ring assay for angiogenesis
摘要
Abstract
Objective:To optimize the method of the mouse aortic ring angiogenesis assay.Methods:The thoracic aorta of C57BL/6 mice were separated and all extraneous fat and connective tissues were re-moved.To remove the vascular adventitia,the aorta was digested with collagenase Ⅱ.The aorta were cut into rings~0.5 mm in width and the rings were embeded in the wells of a 96-well plate coated with type Ⅰcollagen.The embedded rings were fed with 100 μL of DMEM culture medium supplemented with 10%FBS or 2.5%FBS and VEGF to attain a final concentration of 50 ng/mL.Inverted microscope was used to observe microvessel outgrowth of the aortic rings.The endothelial cells were assayed by BS1 lectin-FITC im-munofluorescence.Results:The number of microvessels of the aortic rings in the 96-well plate was more than those in 24-well plate(t=-6.000,P<0.05).The number of microvessels of the aortic rings cultured with DMEM+10%fatal bovine serum was more than those cultured with DMEM+2.5%fatal bovine serum or DMEM+2.5% fatal bovine serum+VEGF.Microvessel outgrowth of the aortic rings started at 2 to 4 days and reached the peak at day 6 to 8 using the modified method.Conclusion:A great quantity,purity and high survival rate of the mouse aortic ring model of angiogenesis can be effectively obtained by coating plates with type Ⅰ collagen and enhancing the concentration of FBS.关键词
主动脉环/血管新生/组织培养Key words
aortic ring/angiogenesis/tissue culture分类
医药卫生引用本文复制引用
翁嘉懿,严金川,陈瑶,李波,李晓阳,徐源,刘俊..小鼠主动脉环血管新生实验模型的优化[J].江苏大学学报（医学版）,2016,26(3):227-230,4.基金项目
国家自然科学基金资助项目（81170279，81370409）；江苏省科教兴卫工程项目（LJ201116）；江苏省六大人才高峰项目（WS074）；江苏省333高层次人才项目 （）
