口腔颌面外科杂志2016，Vol.26Issue(2)：96-100,5.DOI:10.3969/j.issn.1005-4979.2016.02.004
牙髓细胞和肋软骨细胞共培养形成组织工程软骨的胶原类型分析
Investigation of Engineered Cartilage Formed from Coculture of Costal Chondrocytes and Dental Pulp Cells：a Preliminary Study
殷鹏 1袁冬2
作者信息
- 1. 首都医科大学附属北京潞河医院口腔科，北京 101149
- 2. 首都医科大学口腔医学院老年科，北京 100050
- 折叠
摘要
Abstract
Objective:This preliminary study aimed to explore the collagen types of engineered cartilage formed from co-culturing costal chondrocytes (CCs) and dental pulp cells (DPCs). Methods: Human CCs and human DPCs were mixed at a ratio of 1∶1 to form pellets, and were co-cultured in chondrogenic medium to promote chondrogenic differentiation of DPCs. Then the picric acid/sirius red staining and polarization microscopy were used to explore the collagen types of the engineered cartilage. Results: The formed engineered cartilage was mainly composed of type I collagen, simultaneously including type II and type III collagen. The histological structure of engineered cartilage was similar to that of costal cartilage, temporomandibular joint disc cartilage, and the proliferation layer of condylar cartilage. Conclusion: The engineered cartilage formed from co-culturing CCs and DPCs was similar with fibrous cartilage.关键词
牙髓细胞/肋软骨细胞/共培养/组织工程软骨/类型Key words
dental pulp cells/costal chondrocytes/co-culturing/engineered cartilage/type分类
医药卫生引用本文复制引用
殷鹏,袁冬..牙髓细胞和肋软骨细胞共培养形成组织工程软骨的胶原类型分析[J].口腔颌面外科杂志,2016,26(2):96-100,5.
