河南科技大学学报（医学版）2016，Vol.34Issue(2)：81-83,90,4.DOI:10.15926/j.cnki.issn1672-688x.2016.02.001
小鼠胰岛β细胞（Min6）长链非编码 RNA Meg3绑定 PRC2沉默 Hes1表达
LncRNA Meg3 Silences Hes1 Expression by Binding to PRC2 in Mice Pancreatic βCells(Min6)
摘要
Abstract
Objective To explore the relationship of Meg3 and PRC2 complexes as well as its downstream genes in pancreatic βcells (Min6). Methods qPCR detection Meg3 in localization of Min6 cell nucleus and cytoplasm,we also verified that the relationship of Meg3,Ezh2 as well as its downstream genes Hes1 by RIP,UCSC Genome Browser and CHIP. Results The expression of Hes1 was significantly suppressed by downregulation of Si-Ezh2 and Si-Suz12 using small interfering RNA in Min6 cells.The results of CHIP assays showed that Ezh2 could directly bind to the promoter region of Hes1 and mediate H3K27me3 modification,while transfection of Meg3 and Ezh2 led to reduced Ezh2 and H3K27 binding ability. Conclusion These results indicated that lncRNA Meg3 could bind PRC2 complex in Min6 cells,and Ezh2 could regulate the expression of downstream transcription factor gene Hes1 directly.The discovery would further enrich and improve the gene regulatory network of pancreatic βcells on Meg3 and PRC2 complexes.关键词
长链非编码RNA/胰岛β细胞/Meg3/PRC2/Hes1表达Key words
lncRNA/pancreatic βcells/Meg3/PRC2/Hes1 expression分类
医药卫生引用本文复制引用
胡艳妹,朱亚男,德伟..小鼠胰岛β细胞（Min6）长链非编码 RNA Meg3绑定 PRC2沉默 Hes1表达[J].河南科技大学学报（医学版）,2016,34(2):81-83,90,4.基金项目
安徽省2015年度高校自然科学研究基金重点项目（No． KJ2015 A374）安徽省2015年度高等教育振兴计划重大项目 （）
