河南科技大学学报（医学版）2016，Vol.34Issue(2)：84-86,3.DOI:10.15926/j.cnki.issn1672-688x.2016.02.002
核素锝［99m Tc］对维甲酸诱导骨髓间充质干细胞的干预研究
Intervention Study of Retinoic Acid Induced Bone Mesenchymal Stem Cell Differentiated into Neuron with Nuclide Technetium[99m Tc]
李玮1
作者信息
- 1. 河南科技大学第一附属医院，河南洛阳 471003
- 折叠
摘要
Abstract
Objective To study the impact of [99m Tc]to bone mesenchymal stem cells (MSCs)induced by retinoic acid to differentiate into neuron-like cell. Methods MSCs were isolated and purified by density gradient centrifugation and adherent culture,and then induced into neuron-like cell by 30 μmol·L -1 retinoic acid.Biologcial effects of neuron-like cell were determined after [99m Tc]interfered in the differentiation of MSCs. Results After the interference of 740、1 1 10 MBq·mL -1 [99m Tc]for 6 hours,cell survival rate was(96.03 ±2.21 )% and (94.47 ±2.12 )% respectively,and Nestin and NSE were expressed in the progress of 740 MBq · mL -1 [99m Tc ] interfering in the differentiation of MSCs. Conclusion The cell viability and differentiation of MSCs were not affected by the conventional dose of [99m Tc]in vitro.关键词
99m Tc/骨髓间充质干细胞/神经前体细胞/神经元Key words
99m Tc/bone mesenchymal stem cell/nerve precursor cell/neuron分类
医药卫生引用本文复制引用
李玮..核素锝［99m Tc］对维甲酸诱导骨髓间充质干细胞的干预研究[J].河南科技大学学报（医学版）,2016,34(2):84-86,3.
