林业科学2016，Vol.52Issue(5)：71-80,10.DOI:10.11707/j.1001-7488.20160509
杞柳不同品种对铅的积累、耐性及叶片元素原位微区分布特征
Variations in Lead Accumulation and Tolerance in Different Varieties of Salix integra and in Situ Distribution of Elements in Leaves under Pb Stress
摘要
Abstract
[Objective]This study aimed to evaluate phytoremediation potential of different Salix integra varieties in the remediation of lead contaminated soils and to provide information for clarifying the Pb toxicity to willow trees. [Method]To investigate the variation of Pb tolerance and accumulation in S. integra,ahydroponic experiment with three cultivated varieties of S. integra including Weishanhu,Yizhibi and Dahongtou was conducted and the seedlings were treated with three Pb concentrations ( CK: 0 μmol·L -1 ,T1: 100 μmol · L -1 ,T2: 200 μmol · L -1 ) . [Result]Compared to the control,the height growth and biomass accumulation of seedlingsof all three varieties subjected to Pb treatments were inhibited significantly ( P <0 . 05 ) . The seedling height growth showed a significant decrease with the increase of Pb concentration,whereas no significant changes were observed in the dry weight of the aboveground parts and the root with 100 μmol·L -1 and 200 μmol·L -1 of Pb treatments. The Pb tolerance of the three varieties differed depending on the Pb concentration and the Pb accumulation varied in different organs. All three varieties showed high tolerance index ( TI) under both Pb treatments,of which Weishanhu exhibited the highest TI,followed by Yizhibi,and Dahongtou had the lowest TI. The accumulation of Pb in different tissues was from high to low: root > stem > twig > leaf. Based on the calculation of translocation factor ( TF) for the aboveground tissues,an extremely low TF was found in wood,new shoots and leaves,indicating that Pb sequestration was mainly restricted in roots. The aboveground Pb was mainly reserved in stem. In this study,Pb and other mineral elements distribution in leaf tissues were also investigated by synchrotron radiation X-ray fluorescence analysis. The results indicated that Pb concentrations were detected in the middle vein of leaves,which was similar to the distribution of other ions,such as Zn and K. According to Pearson correlation analysis, the accumulation of Pb in leaves was positively related with K,Zn,Ca and Fe,indicating the translocation of Pb in leaves may be related to the uptake of these ions.[Conclusion]S. integra can tolerate and accumulate high levels of Pb in the root when exposed to different Pb concentrations,and there is no significant difference in the tolerance to Pb among the three varieties. However,as a good candidates for phytoremediation,Weishanhu exhibits better growth and biomass accumulation than the other two varieties,thus could be recommended for phytostablization of Pb in contaminated areas.关键词
杞柳/铅/耐性和积累/同步辐射 -X-射线荧光Key words
Salix integra/lead/tolerance and accumulation/synchrotron radiation X-ray fluorescence分类
农业科技引用本文复制引用
王树凤,施翔,田生科,孙海菁,杨肖娥,陈益泰,刘婷..杞柳不同品种对铅的积累、耐性及叶片元素原位微区分布特征[J].林业科学,2016,52(5):71-80,10.基金项目
国家自然科学基金青年科学基金项目(31400526) （31400526）
中央级公益性科研院所基本科研业务费专项资金(CAFYBB2014QB035) （CAFYBB2014QB035）
浙江省科技厅公益技术研究发展项目(2016C33043)。 （2016C33043）
