农业环境科学学报2016，Vol.35Issue(5)：878-885,8.DOI:10.11654/jaes.2016.05.009
砷胁迫下吲哚乙酸对不同砷富集能力植物根系形态和生理的影响
Effects of indole-3-acetic acid on morphologic and physiological characteristics of root systems of plants with different arsenic-accumulating abilities under As stress
摘要
Abstract
A hydroponic experiment was conducted to investigate the effects of indole-3-acetic acid(IAA)on growth, root morphology, root activity, root ATPase activity in cytoplasmic membrane, root antioxidative enzymes(superoxide dismutase SOD, catalase CAT and proxidase POD), malondialdehyde(MDA, a peroxidation product of membrane lipids)content, and As accumulation in plants with different As-accu-mulating abilities. Arsenic hyperaccumulator(Pteris cretica var. nervosa)and non-hyperaccumulator(Pteris ensiformis)were exposed to 2 mg·L-1 As(V)and different concentrations of IAA(0,10,20,40,and 60 mg·L-1)for 14 d. Results showed that biomass of two plants signif-icantly increased in the presence of 20 mg·L-1 IAA, compared with the control. At the 20 mg·L-1 IAA treatment, As concentrations in fronds and petioles, root activity, and root ATPase activity in cytoplasmic membrane of P. cretica var. nervosa increased significantly, and were sig-nificantly higher than those in P. ensiformis. At 20~60 mg·L-1 IAA treatments, the activities of three antioxidative enzymes(CAT, SOD and POD)in roots of P. cretica var. nervosa increased significantly, but the CAT activity decreased and SOD activities increased significantly in roots of P. ensiformis, in comparison with no IAA treatment. The POD activity showed no significant changes in the roots of P. ensiformis. The content of MDA in roots of both plants were significantly decreased by 20~40 mg·L-1 IAA treatments. However, MDA content in the roots of P. cretica var. nervosa was significantly lower than that of P. ensiformis. Correlation analysis showed that As concentrations in roots of P. cretica var. nervosa were positively related with root activity, whereas no correlation was found between root As concentrations and root parameters in P. ensiformis. Therefore, As hyperaccumulation of P. cretica var. nervosa was attributed to its high root activities.关键词
3-吲哚乙酸/超富集植物/砷/根系/根系活力Key words
indole-3-acetic acid/hyperaccumulator/arsenic/root system/root activity分类
资源环境引用本文复制引用
和淑娟,王宏镔,王海娟,赵宾,李勤椿..砷胁迫下吲哚乙酸对不同砷富集能力植物根系形态和生理的影响[J].农业环境科学学报,2016,35(5):878-885,8.基金项目
国家自然科学基金项目（31360132）；云南省中青年学术和技术带头人后备人才项目（2012HB007）；昆明理工大学人才培养基金项目（KKZ3201222024）；国家国际科技合作专项 （）
