热带亚热带植物学报2016，Vol.24Issue(3)：259-266,8.DOI:10.11926/j.issn.1005-3395.2016.03.003
福建泰宁野生铁皮石斛种群的 ISSR 亲缘关系分析
Genetic Relationships among Wild Dendrobium officinale Populations in Taining, Fujian by ISSR
摘要
Abstract
In order to understand the genetic relationships among germplasms of Dendroubium officinale, the genetic relationships and genetic diversity of 34 germplasms of D. officinale were analyzed by ISSR. The results showed that total 78 bands were amplified by 9 ISSR primers with percentage of polymorphic loci at 100%. UPGMA cluster analysis indicated that the similarity coefficient ranged from 0.61 to 0.92, and wild and cultivated D. officinale were divided into 2 groups at level of 0.626. The Nei’s gene diversity (H) and coefficient of gene differentiation (Gst) of wild D. officinale from Fujian Taining was 0.3111 and 0.4609, respectively, which were higher than those of cultivated D. officinale (0.3056 and 0.4204, respectively), indicated that there were high diversity and gene differentiation among Taining D. officianle populations. AMOVA analysis showed that variation coefficients between populations and within populations were 24% and 76%, respectively, which indicated that there was gene flow among different populations. These would provide theoretical basis and reference for efficient protect and utilization of wild D. officinale germplasms.关键词
铁皮石斛/亲缘关系/ISSR/遗传多样性Key words
Dendrobium officinale/Genetic relationship/ISSR/Genetic diversity引用本文复制引用
江金兰,叶炜,李永清,范志敏,雷伏贵,张锐,周建金..福建泰宁野生铁皮石斛种群的 ISSR 亲缘关系分析[J].热带亚热带植物学报,2016,24(3):259-266,8.基金项目
国家级星火计划项目(2014GA720032)；福建省种业创新与产业化工程项目(FJZZZX-1540)；福建省三明市科技计划项目(2014-N-3)；三明市农业科学研究院院立项目(2013-Y-01)资助This work was supported by the China Spark Program (Grant No.2014GA720032), the Seed Industry Innovation and Industrialization Project in Fujian Province (Grant No. FJZZZX-1540), the Sanming Science and Technology Plan Projects in Fujian Province (Grant No.2014-N-3), and the Program of Sanming Institute of Agricultural Science (Grant No.2013-Y-01) （2014GA720032）
