热带亚热带植物学报2016，Vol.24Issue(3)：273-279,7.DOI:10.11926/j.issn.1005-3395.2016.03.005
内源抗坏血酸对水稻种子萌发及幼苗生长的影响
Effects of Endogenous Ascorbic Acid on Seed Germination and Seedling Growth of Rice
摘要
Abstract
The aim was to understand the function of endogenous ascorbic acid in rice (Oryza sativa) seeds. The homozygote transgenic lines GO-2 with the up-regulated GLDH (the key enzyme that catalyzes the final step of Asc biosynthesis in plants gene) and GI-2 with the down-regulated GLDH had been established from the cultivar‘Zhonghua 11’ (ZH-11). The effect of endogenous ascorbic acid on seed germination and seedling growth of rice was studied among ZH-11, GO-2 and GI-2. The results showed that the germination rate of GO-2 was 6% and 60% higher than that of wild type (ZH-11), and GI-2, respectively. After treatment with 1 mmol L–1 exogenous ascorbic acid, the germination rate of GI-2 increased by 22%, but no significant effect was observed in that of GO-2 and the wild type (ZH-11). The root length, plant height, tiller number and fresh weight of seedling in GO-2 were higher than those in ZH-11 or GI-2. The expression of GLDH gene in GO-2 leaves was higher than that in ZH-11 or GI-2, too. So, these indicate ascorbic acid plays an important role in maintaining seed vigor and improving germination and seedling growth of rice.关键词
抗坏血酸/GLDH 基因/水稻/种子萌发/幼苗生长Key words
Ascorbic acid/GLDH gene/Oryza sativa/Seed germination/Seedling growth引用本文复制引用
张启雷,刘强,高辉,陆俐娜,彭长连..内源抗坏血酸对水稻种子萌发及幼苗生长的影响[J].热带亚热带植物学报,2016,24(3):273-279,7.基金项目
国家自然科学基金项目(31270287,31570398)；广东省自然科学基金重点项目(2015A030311023)资助This work was supported by the National Natural Science Foundation of China (Grant No.31270287,31570398), and the Key Program of Natural Science Foundation of Guangdong Province (Grant No.2015A030311023) （31270287,31570398）
